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An episomal fixative

A free gene and fixative technology, applied in the field of special non-invasive gene blood collection tube additives, can solve problems such as miscarriage

Active Publication Date: 2021-04-13
安徽信灵检验医学科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this invasive examination is a routine medical practice, it has a great risk of miscarriage

Method used

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  • An episomal fixative
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Embodiment Construction

[0015] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0016] A kind of episomal gene fixative, is made up of the raw material of following parts by weight (%):

[0017]

[0018]

[0019] A method for preparing a free gene fixative solution, comprising the following steps: subjecting the above-mentioned raw materials by weight to ultrasound for 20 minutes, requiring a working frequency greater than 40KHZ, and a water temperature of 30°C, and filtering through a 0.1μ membrane to obtain a solution that is the free gene Fixati...

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Abstract

The invention discloses an epigenetic fixative solution, which is composed of the following raw materials in parts by weight (%): sodium citrate 2.6-2.7; citric acid 0.3-0.4; glucose 3-3.5; adenine 0.02-0.04; sodium dihydrogen phosphate 0.2-0.3; sodium dimercaptopropanesulfonate 0.75-0.85; paraformaldehyde 3.85-4.20; glycine 4.85-5.15; trehalose 0.1-0.2; glutaraldehyde 1.85-2.15; water 80.51-82.48. The cell-free gene fixative solution of the present invention protects the genes of blood samples by low temperature or chemical medium in the medical testing field; the present invention adopts the comprehensive technology of protecting intracellular genes and fixing cell-free gene fragments for the first time. The free gene fixative solution of the invention protects the integrity of the cell membrane in the specimen, prevents the genes in the nucleated cells from escaping; degrades and inhibits the activity of gene enzymes, so that the cell-free free genes can be stored in the environment of 2-8°C Within 15 days, there was no significant variation in the content of episomal genes.

Description

technical field [0001] The invention relates to laboratory medicine genetic laboratory: a special non-invasive gene blood collection tube additive for the collection, transportation and preservation of pregnant women's peripheral blood fetus cell-free gene prenatal screening samples, specifically a cell-free gene fixative. Background technique [0002] The current research on clinical medical genetic testing shows that no one is perfect genetically, and each person will carry 30 genetic disease gene defects on average. Therefore, research to find genetic etiology, determine detection markers and therapeutic targets, carry out detection and analysis of genetic disease genes, and provide genetic etiology consultation and scientific research services has extensive clinical application value. With the rapid development of gene detection technology in clinical testing, there are now high-throughput gene detection technologies: QPCR, ddPCR, gene chip and other technologies. The N...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/125
Inventor 王之侃胡圆圆金黄根吴向阳
Owner 安徽信灵检验医学科技股份有限公司
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