Mixed lactic acid bacteria preparation for wrapping silage in alpine pastoral regions

A technology of lactic acid bacteria and wrapping, applied in bacteria, application, animal husbandry and other directions, can solve the problems of advancing the phenological period of forage grass and increasing the loss of silage, and achieve the effect of improving fermentation quality, improving digestibility and producing more acid.

Inactive Publication Date: 2018-03-23
SICHUAN ACAD OF GRASSLAND SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of silage production, low temperature often inhibits the fermentation of lactic acid bacteria and increases the loss of silage
At present, the quality of silage has been impr

Method used

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  • Mixed lactic acid bacteria preparation for wrapping silage in alpine pastoral regions
  • Mixed lactic acid bacteria preparation for wrapping silage in alpine pastoral regions
  • Mixed lactic acid bacteria preparation for wrapping silage in alpine pastoral regions

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Experimental program
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Embodiment Construction

[0026] 1 Materials and methods

[0027] 1.1 Isolation of lactic acid bacteria

[0028] A total of 86 samples of natural silage (old awn wheat and oats) were collected in the alpine pastoral areas of Northwest Sichuan, put into sterile ziplock bags with sterile tweezers, sealed, transported back to the laboratory in a car refrigerator, and stored at -80°C for later use.

[0029] Thaw frozen samples at room temperature on an ultra-clean workbench, take 5g of samples into a 250mL sterile Erlenmeyer flask, add 45mL of 0.9% sterile saline, seal with plastic wrap, shake for 2-3 hours, filter with sterile gauze, and take 1m of the filtrate Carry out serial dilution, take 10 respectively -3 、10 -4 、10 -5 1mL of dilution sample solution was spread in sterile medium. 3-5 flat plates (Φ10cm) were coated for each dilution. The medium was MRS medium, CaCO 3 + MRS compound medium, M17 medium and GYP medium (all mediums are provided by Chengdu Litian Century Technology Co., Ltd., the s...

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Abstract

The invention discloses a mixed lactic acid bacteria preparation for wrapping silage in alpine pastoral regions. The mixed lactic acid bacteria preparation is prepared from lactobacillus plantarum with a preservation number of CGMCC No.14117, lactobacillus paracasei with a preservation number of CGMCC No.14116, and ethanol-resistant pediococcus with a preservation number of CGMCC No.14183. After the mixed lactic acid bacteria preparation is used, the contents of lactic acid and total acid of the silage are higher than those of silage wrapped by separately using the components of the mixed lactic acid bacteria preparation and the existing commercially available products, and the pH value, the number of harmful microorganisms, propionic acid content and a ratio of ammoniacal nitrogen to total nitrogen of the silage are lower than those of the silage wrapped by separately using the components of the mixed lactic acid bacteria preparation and the existing commercially available products; the aerobic stability is improved, the fermentation quality of the silage is further improved, the crude protein content and in vitro dry matter digestibility of the silage are increased, and the silage modulation can be prolonged to mid-August.

Description

technical field [0001] The invention belongs to the field of animal husbandry, and in particular relates to a mixed lactic acid bacteria preparation for wrapping silage in alpine pastoral areas. Background technique [0002] With the development of animal husbandry, lactic acid bacteria preparations are more and more widely used in silage. A large number of studies have shown that the effect of adding lactic acid bacteria varies with the type of metabolism, the characteristics of raw materials and the climate environment. The quantity and composition of lactic acid bacteria attached to forage grass in cool and cool regions were different from those in temperate and tropical regions. Commercial lactic acid bacteria currently used are mainly derived from temperate environments, and some studies are aimed at screening dominant lactic acid bacteria adapted to silage preparation in tropical areas. [0003] Wrapped silage is preserved in a similar manner to most conventional sil...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18C12R1/25C12R1/225C12R1/01
CPCA23K30/18C12N1/20A23V2400/165A23V2400/169A23V2400/41
Inventor 李平白史且沈益新李达旭游明鸿张玉苟文龙
Owner SICHUAN ACAD OF GRASSLAND SCI
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