A kind of improved mycobacterium fluorescent staining solution and staining method

Abstract

The invention discloses an improved mycobacterium fluorescent dyeing solution. The dyeing solution consists of an oxidizing solution, a primary dyeing solution, a decolorizing solution and a secondarydyeing solution, wherein the oxidizing solution adopts a H2O2 solution or H5IO6 solution, and the primary dyeing solution adopts a coumarin solution. The improved mycobacterium fluorescent dyeing solution provided by the invention is dyed after being oxidized by the oxidizing solution, and has the advantages of long storage time, bright color of a dyed sheet, high contrast and no toxicity or harm.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an improved mycobacterium fluorescent staining solution and a staining method. Background technique [0002] Mycobacterium tuberculosis is a kind of bacteria that exists in tuberculosis patients. Finding acid-fast staining-positive Mycobacterium tuberculosis is still one of the most effective and accurate methods for diagnosing tuberculosis. In the 1880s, Ziehi and Neelsen jointly invented the acid-fast staining method for mycobacteria. In the following 100 years, many scholars in China have made many improvements to this method. The method has good sensitivity, good contrast, good sensitivity, and high positive rate. It is undoubtedly the best clinical staining method. But this staining method has serious flaws: [0003] 1. The high toxicity and strong carcinogenicity of auramine O in the staining solution limit its promotion; [0004] 2. The fluorescence in the staining sol...

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Problems solved by technology

But this staining method has serious flaws:

[0003] 1. The high toxicity and strong carcinogenicity of auramine O in the staining solution limit its promotion;

[0004] 2. The fluorescence in the staining solution is easily attenuated and the smears cannot be preserved. The stained smears must be observed immediately, otherwise false negative results or inconsistent conclusions of various clinical units will be caused;

[0005] 3. This kind of staining solution needs to be stored away from light. Exposure to light and repeated opening will easily lead to the failure of the staining solution, which is not conducive to the commercialization of the staining solution and the standardization of clinical staining methods, so it has not been well promoted.

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