Method and system for obtaining magnetic bead recognition threshold, and method and system for recognizing magnetic bead
A technology for identifying thresholds and magnetic beads, applied in the field of identifying magnetic beads, can solve problems such as poor applicability, subjective factors, inaccuracy, etc., and achieve a wide range of applications.
Inactive Publication Date: 2018-04-03
GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
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[0004] The purpose of the present invention is to provide a method and system that can quickly and accurately obtain the magnetic bead recognition threshold, aiming to solve the technical problems of poor applicability, inaccuracy, and subjective factors in the solutions in the prior art
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[0066] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0067] The present invention proposes a first embodiment. like figure 1 As shown, a method for obtaining a magnetic bead recognition threshold comprises the following steps:
[0068] S01, the mean value calculation step, according to the preset threshold, classify all the pixels in the preset area of the high-throughput gene sequencing image into the magnetic bead pixel point set and the background pixel point set, and then calculate all the pixels in the magnetic bead pixel point set The average value of the point signal value, the average value of the magnetic bead pixel point is obtained, and the average value of the signal value of all pixel points in the background pixel point set is calculated to obtain the average value of the background ...
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The invention relates to the field of high-throughput gene sequencing and provides a method and system for obtaining a magnetic bead recognition threshold, and a method and system for recognizing a magnetic bead. In the invention, all pixels in a preset region of a high-throughput gene sequencing image are divided into two types according to a preset threshold, then average values of signal valuesof all the pixels in the two types are calculated respectively, and re-classification is made based on the average values of the two types; and the above process is repeated for a preset number of times to finally obtain a magnetic bead recognition threshold. According to the invention, the adaptive acquisition of the magnetic bead recognition threshold of the high-throughput gene sequencing image is quickly and accurately realized, the application range is wide, and then the magnetic bead in the high-throughput gene sequencing image can be quickly and accurately recognized.
Description
technical field [0001] The present invention relates to the field of high-throughput gene sequencing, and more specifically, relates to a method and system for obtaining a magnetic bead recognition threshold, and a method and system for identifying magnetic beads. Background technique [0002] Identifying magnetic beads in high-throughput gene sequencing images is an important step in the process of obtaining high-throughput gene sequencing results, and the quality of the identification results is directly related to the quality of high-throughput gene sequencing results. In the prior art, operators often determine the magnetic bead recognition threshold through experience, and then use the magnetic bead recognition threshold to perform magnetic bead recognition on high-throughput gene sequencing images, but high-throughput gene sequencing images are affected by sample preparation, Influenced by the conditions during shooting and the operating status of the equipment, the ex...
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Login to View More IPC IPC(8): G06K9/00
CPCG06V40/10G06V2201/04
Inventor 盛司潼冀高
Owner GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD