sweet potato stem 60 co-γ-ray radiation mutation breeding method

A γ-ray, sweet potato technology, applied in the agricultural field, can solve the problems of low genetic diversity, narrow genetic basis, and few breakthrough varieties, and achieve the effects of broadening the genetic basis, enriching genetic variation, and shortening the breeding period

Active Publication Date: 2019-12-10
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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Problems solved by technology

Many research results at home and abroad show that the overuse of core parents leads to low genetic diversity among sweet potato varieties, narrow genetic basis, and fewer breakthrough varieties bred (“Wang Lianjun, Lei Jian, Su Wenjin et al. 2005-2014 sweet potato varieties Analysis of National Appraisal Situation. Hubei Agricultural Science. 2015, Volume 54, Issue 12")

Method used

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  • sweet potato stem  <sup>60</sup> co-γ-ray radiation mutation breeding method
  • sweet potato stem  <sup>60</sup> co-γ-ray radiation mutation breeding method
  • sweet potato stem  <sup>60</sup> co-γ-ray radiation mutation breeding method

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Embodiment 1

[0031] Embodiment 1, sweet potato stem segment 60 Co-γ-ray Radiation Mutation Breeding Method

[0032] 1. Collection of seedlings

[0033] Cut the seedlings before 9 o'clock in the morning and after 17:00 in the afternoon on cloudy days or sunny days, and select sweet potato seedlings that are free from diseases and insect pests and grow robustly. The length is 30-40 cm, and each sweet potato seedling has 18-35 side buds. Cut off all the old leaves on the cutting seedlings, and tie them with plastic ropes or rubber bands to obtain seedlings, with 20-50 plants per bundle. Spread it out and place it in a temporary storage place, which requires a cool, ventilated, dry and dark place.

[0034] 2. Mutagenesis treatment

[0035] The 300 seedlings after 1 treatment were divided into 3 groups, 100 plants in each group, and the doses of 50Gy, 100Gy, and 150Gy were used respectively. 60 Co-γ-ray irradiation treatment (dose rate 2Gy / min, 50Gy, 100Gy, 150Gy radiation treatment time is...

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Abstract

The invention discloses a stem-segment <60>Co-gamma ray radiation mutation breeding method for sweet potato. The method provided by the invention comprises the steps of seedling collection, mutation treatment, transplanting and management after transplanting, and continuous cutting propagation and artificial selection of mutant individuals. According to the invention, a treated material is a stemsegment (comprising terminal buds and a great amount of lateral buds), so large quantities of materials can be treated by one time, and the breeding period is effectively shortened; through a vegetative propagation manner of continuous cutting, generation of a chimera is overcome, and stable and uniform variant plants are obtained; meanwhile, by adoption of <60>Co-gamma ray radiation for mutationof the stem segment of the sweet potato, rich genetic variation can be created; the genetic basis of the sweet potato is broadened; a simple, convenient and highly-efficient method is provided for breeding of new varieties of the sweet potato; and the genetic basis of germplasm resources of the sweet potato is broadened.

Description

technical field [0001] The invention belongs to the field of agricultural technology, in particular to a sweet potato stem section 60 Co-γ-ray radiation mutation breeding method. Background technique [0002] Sweet potato (Ipomoea batatas (L.) Lam.) is an important root crop for food, feed, industrial raw materials and new energy in the world. my country is the largest sweet potato producer in the world, with an annual planting area of ​​5.5 million ha 2 , the yield of fresh potato is about 1.2×10 8 t, accounting for 60% and 85% of the world's total sweet potato planting area and total production, respectively. Sweet potato is highly heterozygous genetically, incompatibility of intraspecific and interspecific hybridization, lack of genetic resources, narrow genetic basis, serious damage from diseases, insect pests, and viral diseases severely restrict the genetic improvement of sweet potato varieties ("Wang Lianjun, Lei Jian, Su Wenjin et al. . Isolation and identificatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 王连军杨新笋柴沙沙雷剑苏文瑾王崇
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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