Palmtop pyrosequencing system based on digital microfluidic technology

Abstract

The invention discloses a palmtop pyrosequencing system based on digital microfluidic technology, and belongs to the technical field of digital microfluidics. A digital microfluidic chip, an electromagnetic module, and an integrated circuit are connected, and the integrated circuit and a chemiluminescent collection module are connected to a computer. According to the palmtop pyrosequencing systembased on digital microfluidic technology, a printed circuit board is used as a chip substrate, and based on an on-chip dielectric wetting principle, the electrifying sequence of electrodes that are located on the chip is regulated to achieve liquid drop generation, transportation, mixing and splitting in a sequencing process. The system is easy to operate and is miniaturized, and the produced optical signal can be fast rapidly read, so that single base on the chip is measured. The system is simple in structure, low in cost, easy to integrate, and high in detection sensitivity, the sequencing cost is greatly reduced, and the system can be widely applied to the field of pyrosequencing-based gene detection.

Description

technical field [0001] The invention belongs to the technical field of digital microfluidics, in particular to a handheld pyrosequencing system based on digital microfluidics technology. Background technique [0002] Since the completion of the Human Genome Project in 2003, DNA sequencing technology has made tremendous progress. Many of the resulting methods are commercially available and have the ability to rapidly sequence entire genomes. At this stage, the use of DNA sequencing technology to complete the entire genome sequencing has become a "routine" in life science research. However, as the application environment of DNA sequencing becomes more and more diverse, it has become impossible to expect any single sequencing platform to meet all usage requirements. Therefore, at this stage, it is more necessary to customize the platform to meet the specific needs and usage environments of various applications. Then the DNA sequencing platform with lower throughput, cheaper,...

AI Technical Summary

Problems solved by technology

However, the limitations of conventional microfluidic chips are also very obvious: the structure is complex and the processing is difficult; mechanical pumps and valves are required to be used together, and integration is difficult; the precise control of multiple reagent positions and reaction time is slightly weak

This series of unresolved problems means that integrated microfluidic chips for DNA sequencing have not yet reached maturity

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