Composite probiotic composting agent as well as preparation method and application thereof

A compound probiotic and decomposing agent technology, applied in the biological field, can solve the problems of manure heat cannot be dissipated in time, low nitrogen content of manure, etc., achieve the goal of inhibiting common pathogenic bacteria, high survival rate of viable bacteria, and increasing germination rate Effect

Active Publication Date: 2018-05-04
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned technical problems, this application proposes a compound probiotic decomposing agent and its preparation method and application to solve the problem that the heat cannot be dissipated in time during the fermentation process of manure and the nitrogen content in manure is low.

Method used

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  • Composite probiotic composting agent as well as preparation method and application thereof
  • Composite probiotic composting agent as well as preparation method and application thereof
  • Composite probiotic composting agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Preparation of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, Bacillus licheniformis BL-S and preparation of compound probiotic decomposing agent

[0054] In step 1, Lactobacillus plantarum C2, Bacillus subtilis, Lactobacillus plantarum and Bacillus licheniformis are respectively subjected to primary fermentation to obtain corresponding primary seed culture solutions.

[0055] The fermentation conditions of the primary fermentation include: Lactobacillus plantarum C2, Bacillus subtilis, Lactobacillus plantarum and Bacillus licheniformis respectively inoculum 2%-10% (v / v), temperature 33-37°C, rotating speed 50-100r / Cultivate under the condition of m for 18-24h; preferably, cultivate for 20-22h under the conditions of inoculum size 5%-8% (v / v), temperature 35-36°C, and rotation speed 60-80r / m.

[0056] The number of effective viable bacteria of Lactobacillus plantarum C2 in the first-level seed culture solution is ≥ 2×10 9 CFU / g, effe...

Embodiment 2

[0109] Embodiment 2 Preparation of compound lactic acid probiotics

[0110] According to the same method as in Example 1, the four strains of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, and Bacillus licheniformis BL-S were respectively subjected to primary, secondary seed cultivation, and tertiary seed cultivation. Fermentation culture, respectively centrifuged to obtain the bacterial cells of these four strains, respectively mixed the bacterial cells with the protective agent solution according to the mass ratio of 1:5, 1:8, 1:5, 1:10 to obtain the bacterial suspension, and the The bacterial suspension was freeze-dried to obtain bacterial agents respectively; the bacterial agents of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, and Bacillus licheniformis BL-S were The ratio of 1:1:1:2 is mixed, and the dilution carrier is added for compounding to obtain a compound probiotic decomposing agent, and the to...

Embodiment 3

[0111] Embodiment 3 Preparation of compound lactic acid probiotics

[0112] According to the same method as in Example 1, the four strains of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, and Bacillus licheniformis BL-S were respectively subjected to primary, secondary seed cultivation, and tertiary seed cultivation. Fermentation culture, respectively centrifuged to obtain the cells of these four strains of bacteria, the cells were mixed with the protective agent solution according to the mass ratio of 1:7, 1:10, 1:5, 1:8 to obtain a bacterial suspension, and the The bacterial suspension was freeze-dried to obtain bacterial agents respectively; the bacterial agents of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, and Bacillus licheniformis BL-S were The ratio of 1:4:1:6 is mixed, and the dilution carrier is added for compounding to obtain a compound probiotic decomposing agent, and the total number of viab...

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Abstract

The invention provides a composite probiotic composting agent as well as a preparation method and application thereof. The composite probiotic composting agent is prepared from a lactobacillus plantarum P-8 bacterium, a lactobacillus plantarum C2 bacterium, a bacillus subtilis HM-66 bacterium, a bacillus licheniformis BL-S bacterium and a dilution carrier which are mixed and compounded, the numberof viable bacteria of the two lactobacillus plantarum bacteria is greater than or equal to 2*10<11>CFU / g, and the numbers of viable bacteria of the two bacillus subtilis bacteria are both greater than 1*10<11>CFU / g. The composite probiotic composting agent of which the viable bacterium number is 16 billion CFU / g is adopted to compost 200 tons of an excrement fertilizer with sheep manure as a mainraw material for 60 days, the harmless treatment effect of the excrement fertilizer is improved, the heating speed of the fertilizer can be increased, and the heat preservation capability of the fertilizer is improved; the odor of the excrement fertilizer is rapidly lightened, and the color and the texture of the excrement fertilizer are improved; the contents of organic matters and nutrients such as nitrogen, phosphorus and potassium in the excrement fertilizer are increased; the seed sprouting rate of the excrement fertilizer is increased; and the contents of lactic acid bacteria and probiotic bacillus subtilis in the excrement fertilizer in the composting process are increased, and the total bacterial colony number can be reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a compound probiotic decomposing agent and a preparation method and application thereof. Background technique [0002] With the continuous increase of large-scale and intensive livestock and poultry farms, the discharge of livestock and poultry manure is also gradually increasing. Most of the livestock and poultry manure is not used as a beneficial resource, but is discharged arbitrarily or Discarded into the environment, a large part of resources have become a source of environmental pollution, which not only has a considerable negative impact on the environment, but also has an adverse impact on the environment and residents' lives. [0003] In fact, large-scale livestock and poultry manure is a huge resource pool. Unlike many industrial pollution sources, livestock and poultry manure contains rich organic substances, and also contains a large amount of nitrogen, phosph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C05F17/00C12R1/125C12R1/25C12R1/10
CPCC05F3/00C05G3/00C12N1/20C05F17/20C05F7/00C05F11/08Y02W30/40
Inventor 黄卫强张永军
Owner INNER MONGOLIA SCI PLUS BIOTECH
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