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A compound probiotic decomposing agent and its preparation method and application

A technology of compounding probiotics and decomposing agent is applied in the biological field, which can solve the problems of low nitrogen content in manure and inability to disperse the heat of manure in time, and achieve the effects of reducing the total number of bacterial colonies, increasing the germination rate and diluting odor.

Active Publication Date: 2021-08-27
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned technical problems, this application proposes a compound probiotic decomposing agent and its preparation method and application to solve the problem that the heat cannot be dissipated in time during the fermentation process of manure and the nitrogen content in manure is low.

Method used

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  • A compound probiotic decomposing agent and its preparation method and application
  • A compound probiotic decomposing agent and its preparation method and application
  • A compound probiotic decomposing agent and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Preparation of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, Bacillus licheniformis BL-S and preparation of compound probiotic decomposing agent

[0054] In step 1, Lactobacillus plantarum C2, Bacillus subtilis, Lactobacillus plantarum and Bacillus licheniformis are respectively subjected to primary fermentation to obtain corresponding primary seed culture solutions.

[0055] The fermentation conditions of the primary fermentation include: Lactobacillus plantarum C2, Bacillus subtilis, Lactobacillus plantarum and Bacillus licheniformis respectively inoculum 2%-10% (v / v), temperature 33-37°C, rotating speed 50-100r / Cultivate under the condition of m for 18-24h; preferably, cultivate for 20-22h under the conditions of inoculum size 5%-8% (v / v), temperature 35-36°C, and rotation speed 60-80r / m.

[0056] The number of effective viable bacteria of Lactobacillus plantarum C2 in the first-level seed culture solution is ≥ 2×10 9 CFU / g, effe...

Embodiment 2

[0109] Embodiment 2 Preparation of compound lactic acid probiotics

[0110] According to the same method as in Example 1, the four strains of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, and Bacillus licheniformis BL-S were respectively subjected to primary, secondary seed cultivation, and tertiary seed cultivation. Fermentation culture, respectively centrifuged to obtain the bacterial cells of these four strains, respectively mixed the bacterial cells with the protective agent solution according to the mass ratio of 1:5, 1:8, 1:5, 1:10 to obtain the bacterial suspension, and the The bacterial suspension was freeze-dried to obtain bacterial agents respectively; the bacterial agents of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, and Bacillus licheniformis BL-S were The ratio of 1:1:1:2 is mixed, and the dilution carrier is added for compounding to obtain a compound probiotic decomposing agent, and the to...

Embodiment 3

[0111] Embodiment 3 Preparation of compound lactic acid probiotics

[0112] According to the same method as in Example 1, the four strains of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, and Bacillus licheniformis BL-S were respectively subjected to primary, secondary seed cultivation, and tertiary seed cultivation. Fermentation culture, respectively centrifuged to obtain the cells of these four strains of bacteria, the cells were mixed with the protective agent solution according to the mass ratio of 1:7, 1:10, 1:5, 1:8 to obtain a bacterial suspension, and the The bacterial suspension was freeze-dried to obtain bacterial agents respectively; the bacterial agents of Lactobacillus plantarum C2, Bacillus subtilis HM-66, Lactobacillus plantarum P-8, and Bacillus licheniformis BL-S were The ratio of 1:4:1:6 is mixed, and the dilution carrier is added for compounding to obtain a compound probiotic decomposing agent, and the total number of viab...

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Abstract

The application provides a compound probiotic decomposing agent and its preparation method and application. The compound probiotic decomposing agent is composed of Lactobacillus plantarum P-8 bacterial agent, Lactobacillus plantarum C2 bacterial agent, Bacillus subtilis HM-66 bacterial agent, Bacillus licheniformis BL‑S inoculum and dilution carrier are mixed and compounded, and the number of live bacteria of the two Lactobacillus plantarum inoculums is ≥2×10 11 CFU / g, the number of live bacteria of the two Bacillus inoculum agents is ≥1×10 11 CFU / g. Using a compound probiotic decomposing agent with a total viable bacteria content of 16 billion CFU / g to decompose 200 tons of manure with sheep manure as the main raw material for 60 days can improve the effect of harmless treatment of manure, which is manifested as accelerating the heating rate of the fertilizer body. Strengthen the heat preservation capacity of the fertilizer body; quickly desalinate the manure odor, improve the color and texture of the manure; increase the content of organic matter and nitrogen, phosphorus, potassium and other nutrients in the manure; increase the germination rate of manure seeds; Bacillus content, reduce the total number of colonies.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a compound probiotic decomposing agent and a preparation method and application thereof. Background technique [0002] With the continuous increase of large-scale and intensive livestock and poultry farms, the discharge of livestock and poultry manure is also gradually increasing. Most of the livestock and poultry manure is not used as a beneficial resource, but is discharged arbitrarily or Discarded into the environment, a large part of resources have become a source of environmental pollution, which not only has a considerable negative impact on the environment, but also has an adverse impact on the environment and residents' lives. [0003] In fact, large-scale livestock and poultry manure is a huge resource pool. Unlike many industrial pollution sources, livestock and poultry manure contains rich organic substances, and also contains a large amount of nitrogen, phosph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/60C05F17/20C12R1/125C12R1/25C12R1/10
CPCC05F3/00C05G3/00C12N1/20C05F17/20C05F7/00C05F11/08Y02W30/40
Inventor 黄卫强张永军
Owner INNER MONGOLIA SCI PLUS BIOTECH
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