(Pro) resin receptor (P) RR gene and application of inhibitor of gene

A technology of gene and gene expression, which is applied in the determination/testing of microorganisms, medical preparations containing active ingredients, metabolic diseases, etc., and can solve problems such as unclear effects

Active Publication Date: 2018-05-08
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, (P)RR plays an important role in various disorders of glucose and lipid metabolism, such as diabetes and atherosclerosis. production and its role in obesity and fatty liver remain unclear,

Method used

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  • (Pro) resin receptor (P) RR gene and application of inhibitor of gene
  • (Pro) resin receptor (P) RR gene and application of inhibitor of gene
  • (Pro) resin receptor (P) RR gene and application of inhibitor of gene

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Obtain control group mice and experimental group mice and carry out genotype identification

[0053] As experimental mice, C57 mice were used. Alb-cre + / + Male mice were purchased from Nanjing model animals, and the mice specifically expressed Cre recombinase in the liver. (P)RR Flox / WT Female mice were purchased from Nanjing model animals. The mice were transgenic mice with loxP sites at both ends of exon 2 of the (P)RR gene, wherein exon 2 of the (P)RR gene together with its two ends The loxP site is referred to as the Flox fragment or (P)RR flox in the present invention. (P)RR Flox / WT The mouse preparation process includes constructing an expression vector containing (P)RR flox, transfecting mouse embryonic stem cells, performing homologous recombination, screening out cells expressing (P)RR flox, and microinjecting the cells into early stage mice Embryos were transplanted into pseudopregnant mice, and transgenic mice expressing (P)RR flox were obtained throu...

Embodiment 2

[0086] 1. Inhibition of (P)RR expression by tail vein injection of (P)RR liver-specific antisense oligonucleotides (ie, knockdown)

[0087] Common C57 mice were used as the experimental objects. The mice in the blank control group were injected with PBS through the tail vein, the mice in the scrambled control group were injected with the liver-specific scrambled sequence, and the mice in the experimental group were injected with (P)RR liver-specific reaction through the tail vein. The sense oligonucleotide drug was injected once a week, and the injection amount of both the control group and the experimental group was 3 mg / kg / week.

[0088] Wherein, the liver-specific scrambled sequence is: 5'-GGCCAATACGCCGTCA-3'; the sequence of (P)RR liver-specific antisense oligonucleotide is: 5'-AGATATTGGTCCATTT-3'.

[0089] 2. Study the function of the liver (P)RR gene through the obese mouse model induced by high-fat diet.

[0090] The mice in the three groups were all male and 8 weeks o...

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Abstract

The invention discloses a function and application of a (pro) resin receptor [(pro) resin receptor, (P) RR] inhibitor in nonalcoholic fatty livers and fat diseases. Under the background of C57 mice, (P) RR liver specificity knock-out/know-down mice are used as experimental objects, according to a fat mouse model which is induced through high-fat feed, and it is proved through a result that the size of the liver (P) RR knock-out mice is remarkably small, and the fat tissue in the mouse bodies is remarkably reduced; after the mice are fed with the high-fat feed, the increase amplitude of the weight of the mice is low, fat is not generated, the blood glucose content is low, through results of oil red dyeing, liver lipid measurement and the like, it is explained that the liver function of theliver (pro) resin receptor knock-out mice is remarkably better than that of contrasted fatty mice, the fatty liver lesion of the mice is remarkably improved, the stored lipid is remarkably reduced, and the blood glucose content is lowered. Therefore, (P) RR can be used as a medicine target of screening treatment of type-II diabetes, nonalcoholic fatty livers and fat diseases, and the inhibitor of(P) RR can be applied to preparation of medicine for treating type-II diabetes, nonalcoholic fatty livers and fat diseases.

Description

technical field [0001] The invention belongs to the field of gene function and application, in particular to a kind of renin (original) receptor (P) RR gene and its inhibitor as target gene in the preparation of prevention, mitigation and / or treatment of type II diabetes, non-alcoholic fat Drug application in liver and obesity. Background technique [0002] With the Westernization of the diet structure of Chinese residents and the prevalence of more sedentary and less active lifestyles, the number of patients with metabolic syndrome including obesity and diabetes has increased significantly. According to the latest epidemiological survey statistics, the incidence of type 2 diabetes in my country is as high as 11.6%, the prevalence of overweight and obesity is as high as 23%, and the incidence of various lipid metabolism disorders is as high as 12%. The frequent occurrence of this kind of metabolic syndrome has also directly led to a substantial increase in the incidence of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K31/713A61P3/04A61P3/10A61P1/16
CPCG01N33/6872A61K31/713C12Q1/6883G01N2333/705C12Q2600/178
Inventor 卢玺峰何永成谭仑波孙源任丽伟王娜林惠
Owner SHENZHEN UNIV
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