Etv2 and uses thereof

Abstract

Described herein is a method for producing a chimeric non-human animal expressing a human ETV2 gene comprising: a) generating an ETV2 null non-human animal cell, wherein both copies of the non-human ETV2 gene carry a mutation that prevents production of functional ETV2 protein in said non-human animal; b) creating an ETV2 null non-human blastocyst by somatic cell nuclear transfer comprising fusinga nucleus from said ETV2 null non-human animal cell of a) into an enucleated non-human oocyte and activating said oocyte to divide so as to form an ETV2 null non-human blastocyst; c) introducing human stem cells into the ETV2 null non-human blastocyst of b); and d) implanting said blastocyst from c) into a pseudopregnant surrogate non-human animal to generate a chimeric non-human animal expressing human ETV2.

Description

[0001] priority statement [0002] This application claims the benefit of priority to US Provisional Patent Application No. 62 / 127,330, filed March 3, 2015, which is hereby claimed, and which is hereby incorporated by reference in its entirety. [0003] sequence listing [0004] This document incorporates herein by reference the electronic sequence listing text file, which is submitted electronically via EFS-Web. The text file is called "1552048.txt," is 12,288 bytes long, and was created on March 1, 2016. Background technique [0005] Blood is the main organ of the human body. It consists of cells and plasma. The cellular portion includes red blood cells, white blood cells, and platelets. The plasma composition consists of water and dissolved compounds such as proteins, sugars or electrolytes, among others. [0006] Blood formation begins during gastrulation. The first hematopoietic lineage arose in the blood islands of the yolk sac. The differentiation of hematopoieti...

AI Technical Summary

Problems solved by technology

Deletion of the mouse Etv2 gene results in loss of the hematopoietic and endothelial lineages in the yolk sac

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