A kind of low-temperature protection solution of mushroom concentrated bacterial strain and its application
A technology of low-temperature protection and strains, applied in the fields of application, fungi, mushroom cultivation, etc., can solve the problems of easy loss of activity and difficulty in preserving mushroom strains, and achieve the effects of ensuring activity, shortening production cycle, and high production efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] The low-temperature protection solution for the concentrated strain of shiitake mushrooms comprises the following components by weight: 10 wt% of glycerol, 1 wt% of mannitol, 0.002 wt% of 2-chloroethyltrimethylammonium chloride, and the balance is water.
[0023] The method for preserving and inoculating concentrated mushroom strains of shiitake mushrooms comprises the following steps:
[0024] (1) Add the above-mentioned sterile low-temperature protection solution to the concentrated mushroom strain of shiitake mushrooms, stir evenly to obtain the concentrated mushroom spawn of mushrooms, and the addition amount of the low-temperature protection solution is 10wt% of the concentrated mushroom strain of mushrooms;
[0025] (2) seal the processed concentrated strains of shiitake mushrooms obtained in step (1), place them in an environment of 0° C. and store them in the dark for 1 month to obtain the preserved strains; during transportation and mailing, Use a refrigerator ...
Embodiment 2
[0029] The low-temperature protection liquid for the concentrated strain of shiitake mushrooms comprises the following components by weight: 12 wt% of glycerol, 2 wt% of mannitol, 0.001 wt% of 2-chloroethyltrimethylammonium chloride, and the balance is water.
[0030] The method for preserving and inoculating concentrated mushroom strains of shiitake mushrooms comprises the following steps:
[0031] (1) Add the above-mentioned sterile low-temperature protection solution to the concentrated mushroom strain of shiitake mushrooms, and stir evenly to obtain the concentrated mushroom spawn of mushrooms, and the addition amount of the low-temperature protection solution is 3wt% of the concentrated mushroom strain of shiitake mushrooms;
[0032] (2) seal the processed concentrated strains of shiitake mushrooms obtained in step (1), place them in an environment of 2° C. and store them in the dark for 1 month to obtain the preserved strains; during transportation and mailing, Use a ref...
Embodiment 3
[0036] The low-temperature protection liquid for the concentrated strain of shiitake mushrooms comprises the following components by weight: 12 wt% of glycerol, 2 wt% of mannitol, 0.001 wt% of 2-chloroethyltrimethylammonium chloride, and the balance is water.
[0037] The method for preserving and inoculating concentrated mushroom strains of shiitake mushrooms comprises the following steps:
[0038] (1) Add the above-mentioned sterile low-temperature protection solution to the concentrated strain of mushroom, stir evenly to obtain the concentrated strain of mushroom, the addition of the low-temperature protection solution is 5wt% of the concentrated strain of mushroom;
[0039] (2) seal the processed concentrated strains of shiitake mushrooms obtained in step (1), place them in an environment of 6° C. and store them in the dark for 1 month to obtain the preserved strains; during transportation and mailing, Use a refrigerator to keep the temperature low;
[0040] (3) Take out ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More