Bacillus siamese strain and its application

A Bacillus and strain technology, applied to Bacillus siamese strains and their application fields, can solve problems such as undiscovered high-resistance varieties, achieve good application prospects, promote plant growth, and efficiently prevent and control effects.

Active Publication Date: 2021-01-01
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the strong saprophytic ability and wide range of hosts of sheath blight, no high-resistance varieties have been found at home and abroad.

Method used

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  • Bacillus siamese strain and its application
  • Bacillus siamese strain and its application
  • Bacillus siamese strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]1. Initial screening of strains

[0039]Collect 10 fresh feces samples of healthy piglets from all over Jilin Province, weigh 10g samples, add 90mL sterile normal saline to prepare sample suspension, after treatment in 80℃ water bath, take 100μL-2, 10-3, 10-4The dilution was spread on the LB medium plate, each gradient was spread in three parallels, incubated at 37°C for 48 hours, and then the plate streaking method was used to purify the Bacillus strains and store them separately. 122 strains of Bacillus were obtained.

[0040]2. Strain identification

[0041]According to "Berger Bacteria Identification Manual" (eighth edition) and "Common Bacteria System Identification Manual" (edited by Dong Xiuzhu, Cai Miaoying, etc., Beijing: Science Press, 2001.2.), the strain JAASHD The identification of morphological characteristics, culture characteristics and physiological and biochemical characteristics, the specific results are as follows:

[0042]Use LB culture medium at 37°C for 24 hours. Th...

Embodiment 2

[0055]Example 2 Screening of effective antagonistic bacillus against rice sheath blight

[0056]① Preliminary screening: Prepare PDA plates by using the plate confrontation culture method. Use a punch to punch out a 5mm diameter fungus cake on the edges of Bacillus and rice sheath blight, and transplant them on the opposite sides of the plate at a constant temperature of 25°C. Cultivate and observe the inhibitory effect of Bacillus on pathogens daily.

[0057]②Re-screening: Re-screening the selected Bacillus strains with high antagonistic activity, mainly through the temperature tolerance, acid and alkali resistance, and drug resistance tests, and screening the better resistant Bacillus strains for potted control test.

Embodiment 3

[0058]Example 3 Inhibition of Bacillus Siamensis on other pathogens

[0059]The screened Bacillus siamese was screened by the plate confrontation method, which has an inhibitory effect on a variety of plant pathogenic bacteria indicator bacteria (Table 3).

[0060]Table 3 Inhibition of Bacillus siamese JAASHD on other pathogens

[0061]

[0062]

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Abstract

The invention relates to the field of biological control of plant diseases, in particular to a Bacillus siamese strain and application thereof. The strain is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is: CGMCC NO.12903; the preservation time is: August 24, 2016. Using the strain to prevent and control rice sheath blight can avoid potential adverse effects of chemicals used at the present stage on human environment and rice safety. The strain has the characteristics of fast growth, large spore production, wide spectrum of action, strong stress resistance, and promotion of plant growth, so it is a biological control agent with great application prospects.

Description

Technical field[0001]The invention relates to the field of biological control of plant diseases, in particular to a strain of Bacillus siamese and its application.Background technique[0002]As one of the most important food crops in the world, rice is widely grown, especially in my country. The main diseases of rice include rice sheath blight, bacterial leaf blight and rice blast. Rice sheath blight, as an important rice disease all over the world, poses a huge threat to the safety of rice production and income increase. At present, its occurrence area, occurrence frequency, and yield loss are ranked first among the three major rice diseases in some rice areas in my country. In particular, the current lack of rice germplasm resources with high resistance to sheath blight, the increase in the use of nitrogen fertilizers, the increasing planting density, the promotion and planting of dwarf varieties of rice and hybrid rice, etc., have led to the disease The disease is becoming more and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 王景会李达王颖王义生徐文静郑建波牛红红迟燕平苗欣宇李倬林
Owner JILIN ACAD OF AGRI SCI
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