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Application of CA culture medium to culture of strawberry brown pilidium concavum to realize sporulation

A leaf spot fungus and culture medium technology, which is applied in the application field of CA medium for cultivating strawberry brown leaf spot fungus spore production, can solve the problem of low sporulation production, slow sporulation speed of strawberry brown leaf spot fungus, etc. question

Active Publication Date: 2018-05-15
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Strawberry brown leaf spot is an important pathogen that causes strawberries, peonies, peonies, etc. The existing research mainly uses PDA medium to cultivate it, but its general spore production is very low, and the rate of sporulation of strawberry brown leaf spot is limited. Slow enough to carry out related research questions

Method used

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  • Application of CA culture medium to culture of strawberry brown pilidium concavum to realize sporulation
  • Application of CA culture medium to culture of strawberry brown pilidium concavum to realize sporulation

Examples

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Embodiment 1

[0012] Preparation of PDA medium: Peel 200g of potatoes, cut into pieces, cook for 30 minutes until soft and rotten, filter through four layers of gauze, add 20g of glucose, 20g of agar powder in 1L of water, and sterilize at 121℃ for 20min Cool to 60°C and make a flat plate.

Embodiment 2

[0014] Preparation of YDA medium: Yeast powder 5g, glucose 20g, agar powder 20g, dilute in 1L water, sterilize at 121°C for 20min, cool to about 60°C, and make a plate.

Embodiment 3

[0016] SPDA medium: Peel 200g of potatoes, cut into pieces, cook for 30 minutes until soft and rotten, filter through four layers of gauze, add 20g of glucose, 20g of agar powder, add 120ml of freshly squeezed strawberry juice to 1L of water, and pressurize at 121℃ After sterilizing by moist heat for 20 minutes, cool to 60°C and make a flat plate.

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Abstract

The invention relates to application of a CA culture medium to culture of strawberry brown pilidium concavum to realize sporulation. A formula of the CA culture medium is as follows: based on the total volume 1000ml, 100g to 400g of squeezed juice of carrots and 13g to 25g of agar are added.

Description

technical field [0001] The present application relates to the application of CA medium in cultivating sporulation of brown leaf spot fungus of strawberry. Background technique [0002] Strawberry brown leaf spot is an important pathogen that causes strawberries, peonies, peonies, etc. The existing research mainly uses PDA medium to cultivate it, but its general spore production is very low, and the rate of sporulation of strawberry brown leaf spot is limited. Slow enough to carry out relevant research on the problem. Contents of the invention [0003] In order to solve the problems existing in the prior art, one of the present application provides the application of CA medium for cultivating strawberry brown leaf spot fungus (Pilidium concavum) spore production, wherein the formula of the CA medium is as follows: According to the volume of 1000ml, 100g to 400g of carrot juice, and 13g to 22g of agar. [0004] In a specific embodiment, the formulation of the CA medium is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 毕扬张桂军郭巍杨宝东张伟宗召莉
Owner BEIJING UNIV OF AGRI
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