Application of CA culture medium to culture of strawberry brown pilidium concavum to realize sporulation
A leaf spot fungus and culture medium technology, which is applied in the application field of CA medium for cultivating strawberry brown leaf spot fungus spore production, can solve the problem of low sporulation production, slow sporulation speed of strawberry brown leaf spot fungus, etc. question
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 Preparation of PDA medium: Peel 200g of potatoes, cut into pieces, cook for 30 minutes until soft and rotten, filter through four layers of gauze, add 20g of glucose, 20g of agar powder in 1L of water, and sterilize at 121℃ for 20min Cool to 60°C and make a flat plate.
 Preparation of YDA medium: Yeast powder 5g, glucose 20g, agar powder 20g, dilute in 1L water, sterilize at 121°C for 20min, cool to about 60°C, and make a plate.
 SPDA medium: Peel 200g of potatoes, cut into pieces, cook for 30 minutes until soft and rotten, filter through four layers of gauze, add 20g of glucose, 20g of agar powder, add 120ml of freshly squeezed strawberry juice to 1L of water, and pressurize at 121℃ After sterilizing by moist heat for 20 minutes, cool to 60°C and make a flat plate.
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