Application of combination of meropenem and doripenem in resisting of mycobacteria infection

A technology of mycobacteria and meropenem, which is applied in the field of pharmacy, can solve the problems that the application of anti-mycobacterial infection has not been reported.

Inactive Publication Date: 2018-05-18
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] But so far, the combination of meropenem and doripenem has n

Method used

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  • Application of combination of meropenem and doripenem in resisting of mycobacteria infection
  • Application of combination of meropenem and doripenem in resisting of mycobacteria infection
  • Application of combination of meropenem and doripenem in resisting of mycobacteria infection

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Embodiment Construction

[0018] In order to better illustrate the present invention, specific embodiments of the present invention will be described in detail below.

[0019] 1. Expression and purification of β-lactamase (BlaC) in Mycobacterium tuberculosis

[0020] According to the literature (Wang F, Cassidy C, Sacchettini J C. Crystal Structure and Activity Studies of the Mycobacterium tuberculosis β-Lactamase Reveal Its Critical Role in Resistance to β-Lactam Antibiotics [J]. Antimicrobial Agents & Chemotherapy, 2006,50(8):2762-71. )

[0021] (1) Transform the strain of Escherichia coli BL21 (DE3) with the pET28a vector containing the coded BlaC gene, and use LB plate medium (containing 50 mg / L kanamycin) to screen positive clones.

[0022] (2) Pick positive clones on the plate and transfer them to 0.8L LB medium (containing 50mg / L kanamycin) after culturing overnight at 37°C. 600 ) reached 0.8, 0.1 mM IPTG (Isopropyl β-D-Thiogalactoside, Isopropyl β-D-Thiogalactoside) was added and incubated at...

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Abstract

The invention provides a double-drug combination for Mycobacterium Smegmatis. The double-drug combination is meropenem and doripenem, and has remarkable inhibitory activity to the Mycobacterium Smegmatis, and therefore, the provided double-drug combination can be used for preparing a combined drug for mycobacteria, and is expected to become a potential drug for resisting mycobacteria infection.

Description

technical field [0001] The invention relates to the technical field of pharmacy, and specifically relates to the combined use of meropenem and doripenem in anti-mycobacterial infection. Background technique [0002] Mycobacteria belong to the mycolic acid-containing actinomycetes, and the main characteristic is that the cell wall contains a large amount of lipids, mainly mycolic acids. This is closely related to its dyeability, growth characteristics, pathogenicity, and resistance. Generally, it is not easy to be colored. If it is colored by heating or prolonging the dyeing time, it can resist the decolorization of strong decolorizing agent hydrochloric acid ethanol, so it is also called acid-fast bacilli. The bacterium has no flagella, no spores, and does not produce endotoxins and exotoxins. Its pathogenicity is related to the composition of the bacteria. The diseases caused are all chronic and accompanied by granulomas. There are many types, which can be divided into t...

Claims

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Application Information

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IPC IPC(8): A61K31/407A61P31/04A61P31/06
CPCA61K31/407A61K2300/00
Inventor 杨海涛高帆刘祥张晓玲张晴晴陈榕王泽方陈成张瑞
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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