Preparation method of ganoderma sinense and black soybean streptokinase

A technique for thrombolytic enzyme and black bean, which is applied in the field of preparation of Zizhi black bean thrombolytic enzyme, can solve the problems of inability to dissolve thrombus, difficult to control activity, difficult to control dosage, etc., and achieves the solution of difficult persistence, simple equipment and low price. Effect

Inactive Publication Date: 2018-05-18
珠海市微豆生物技术有限公司
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

Streptokinase antibodies are easily produced in the human body, so there is an antagonistic effect, not only the dosage is not easy to control, but also has side effects; although urokinase ha

Method used

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preparation example Construction

[0034] The preparation method of the Zizhi black bean thrombolytic enzyme of the present invention uses the Zizhi black bean as a production strain, uses the black bean as a culture medium, and adopts a solid-state fermentation method to prepare the thrombolytic enzyme from the Zizhi black bean.

[0035] The preparation method of Zizhi black bean thrombus-lytic enzyme of the present invention comprises the solid-state fermentation process of Zizhi black bean and the separation and purification process of thrombus-lytic enzyme.

Embodiment 1

[0037] Solid-state fermentation process of Zizhi black soybean

[0038] 1) Activation of strains:

[0039] Strain Activation Medium (g / L): 200 for potatoes, 200 for carrots, 20 for agar, 1.2 for peptone, 21 for glucose, 0.4 for potassium dihydrogen phosphate, and 0.18 for magnesium sulfate. After sterilizing at 121°C for 30 minutes, make a slope and inoculate Cultivate Zizhi at 26°C for 7-8 days;

[0040] 2), preparation of seed culture medium:

[0041] Seed medium (g / L): black soybean 50, millet 10, sucrose 22, magnesium sulfate 0.5, peptone 5, glucose 10, potassium dihydrogen phosphate 1.5, calcium chloride 0.22; Mix with other materials, add water to 1000mL, boil, inoculate the slant, and shake the flask for fermentation at 26°C for 8 days;

[0042] 3), fermentation culture:

[0043] Fermentation medium (g): black soybean 100, millet 30, sucrose 2.8, glucose 2.8, calcium chloride 0.5, potassium dihydrogen phosphate 1.5, magnesium sulfate 0.5, peptone 0.3, yeast extract ...

Embodiment 2

[0048] The difference between Example 2 and Example 1 is that: the strain activation medium in step 1) of the solid-state fermentation process of Zizhi black beans also includes a small amount of vitamin B1.

[0049]In the present invention, Zizhi is used as a production strain, black beans are used as a medium, and a solid-state fermentation method is adopted. The Zizhi thrombolytic enzyme has strong thrombolytic activity, and there is a serine residue in the active center. It is a serine protease and trypsin. The newly discovered thrombolytic enzyme has the effect of improving immunity, and is suitable for low immunity and the symptoms caused by it. This drink does not contain any additives, and retains the nutrients of black beans, especially the black soybean protein, which also adds a certain effect of enhancing intestinal function and detoxification.

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Abstract

The invention discloses a preparation method of ganoderma sinense and black soybean streptokinase. The preparation method has the advantages that ganoderma sinense serves as a production strain, blacksoybeans serve as culture media, and solid fermentation is adopted; the ganoderma sinense streptokinase has high thrombolytic activity, has serine residues at the active center, belongs to serine protease and trypsin, is newly-found streptokinase with the effect of improving immunity, and is applicable to low immunity and symptoms caused by low immunity; nutrition ingredients of the black soybeans are maintained, intestinal functions of human bodies can be enhanced after the streptokinase is used, and good economic benefit and market prospect are achieved.

Description

【Technical field】 [0001] The invention relates to a preparation method of a thrombolytic enzyme and the field of deep processing of bean fermented products, in particular to a preparation method of a purple lucidum black soybean thrombolytic enzyme. 【Background technique】 [0002] Zizhi, a kind of fungus. Like Ganoderma lucidum. The range of application is very wide, and it can be taken regardless of the weakness of the heart, lungs, liver, spleen, and kidneys. The diseases treated by Zizhi involve respiratory, circulatory, digestive, nervous, endocrine, and immune systems; covering internal, external, gynecological, pediatric, and facial diseases. The chemical composition of Zizhi is fruiting bodies and mycelia, containing proteins, amino acids, sugars, coumarins, steroids and triterpenoids. The fruiting body contains oil, wax, volatile and sublimation substances. Mild, warm, slightly bitter taste, can nourish the middle and strengthen the mind, calm the heart and benef...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12N1/14C12R1/645
CPCC12N1/14C12N9/63
Inventor 程京燕苏保荣高向阳
Owner 珠海市微豆生物技术有限公司
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