Quantitative analysis method of plasma-drug concentration of novel compound WSJ-557 in SD rat plasma

Abstract

The invention relates to a quantitative analysis method of the plasma-drug concentration of a novel compound WSJ-557 in SD rat plasma. The method comprises the following steps of (1) adding methanol and internal standard working fluid into the SD rat plasma after the WSJ-557 intragastric administration; after the vortex, adding ethyl acetate for liquid-liquid extraction; then, performing vortex and centrifugation; taking liquid supernatant; (2) using octadecyl silane bonded silica gel as filling agents for chromatographic column treatment at the column temperature being 40 DEG C, wherein an ultra-high performance liquid chromatography system uses a general binary high-pressure pump and a sample feeding device; using a mixed solution of methanol-ammonium acetate water solution as a flowingphase; performing gradient elution; (3) using an ESI ion source as an ion source and a multi-reaction monitoring mode (MRM) for positive ion detection. The quantitative analysis ions are respectivelyWSJ-557:m/z316.1 to 260.0, m/z237.0 to 194.0; the collision energy is 15eV. The quantitative analysis method has the advantages that the specificity is high; the sensitivity is high; the sampling quantity of samples is small; the pretreatment is simple and fast; the analysis period is short and the like. The method is particularly applicable to the plasma-drug concentration determination and pharmacokinetic study of WSJ-557 in SD rat plasma.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for accurate measurements by measuring low amounts of small samples while also being able to detect very tiny quantities accurately without complicated or expensive equipment that requires high-pressure liquid extraction techniques like solvents. It achieves this by creating an initial solution containing target compounds from blood cells followed by adding specific chemical agents called MIHQAZYE-32, resulting in a complex mixture consisting mostly of these targets molecules. These mixtures are then separated into different fractions before injected back onto the patient's own tissue. By analyzing each fraction separately, it becomes possible to determine how much medicine needs to be given based on its effectiveness against various diseases such as cancer.

Problems solved by technology

The technical problem addressed by this patented technology relates to finding ways to accurately determine how well certain substances such as urate monoferrite or nitrosoureas reduce excessive amounts of uric acids during therapy without causing harmful side effects like other medications used against hyperuricemia symptoms.

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