Optical tweezers optical sheet microscopic imaging device and method

Abstract

The invention relates to a zoom optical-tweezer light-sheet microscopy imaging device and method and belongs to the technical field of optical microscopy imaging and optical manipulation. The device comprises a light-sheet illumination module, a light-sheet detection module, an optical-tweezer focusing module and an optical-tweezer axial focusing module. In a conventional optical-tweezer microscope system, an axial focusing device formed by a polarizing beam splitter, a quarter-wave plate, low-aperture objective lenses, tube lenses and a plane mirror is added, thereby realizing axial movementof an optical tweezer focal plane in an optical tweezer light-sheet microscope, realizing axial movement of a grabbed suspension sample and finishing light-sheet three-dimensional tomographic scanningimaging.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic imaging and optical manipulation, and relates to a microscopic imaging device and method of a zoom optical tweezers optical sheet. It can realize separation and focusing of the optical tweezers microscope system and complete three-dimensional light sheet scanning imaging. Background technique [0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the sample. scan imaging. Adding a zoom lens or DMD to the optical tw...

AI Technical Summary

Problems solved by technology

[0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the sample. scanning imaging

Adding a zoom lens or DMD to the optical tweezers system can change the focus position of the optical tweezers to solve the above problems, but the modulation speed is slow or expensive

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