Zoom optical tweezers confocal microscopy imaging device and method

Abstract

The invention discloses a confocal microimaging device of zooming optical tweezers and a confocal microimaging method of the zooming optical tweezers, and belongs to the technical field of optical microimaging and optical operation. The confocal microimaging device is technically featured by being composed of a confocal lighting module, a confocal scanning module, a confocal detecting module, an optical tweezers focusing module and an optical tweezers axial zooming module. The regular optical tweezers microscopy system is added with an axial zooming device consisting of a polarizing beam splitter, a quarter-wave plate, a low aperture objective lens, a pipe lens and a plane reflective lens; thus the axial movement of the optical tweezers focal face in the co-path optical tweezers confocal microscopy is realized, thereby grabbing a suspending sample and achieving the axial movement, and completing the confocal three-dimensional chromatography scanning imaging. The confocal microimaging device has the advantages of being simple in assembly, quick in zooming and axial chromatography speed, and low in observing cost.

Description

technical field [0001] The invention relates to a microscopic imaging device and method, in particular to a zoom optical tweezers confocal microscopic imaging device and method, which can realize the separation and adjustment of the co-optical path optical tweezers microsystem and complete three-dimensional confocal scanning imaging, which belongs to optical Microscopic imaging and optical manipulation technology. Background technique [0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the sample. scan...

AI Technical Summary

Problems solved by technology

[0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the sample. scanning imaging

Adding a zoom lens or DMD to the optical tweezers system can change the focus position of the optical tweezers to solve the above problems, but the modulation speed is slow or expensive

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