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Cucurbita maxima subgynoecious trait molecular marker and primer pair for identification of cucurbita maxima subgynoecious trait

An Indian squash, molecular marker technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Active Publication Date: 2018-05-29
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant research reports on the molecular markers closely linked to the strong female trait of Indian pumpkin at home and abroad.

Method used

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  • Cucurbita maxima subgynoecious trait molecular marker and primer pair for identification of cucurbita maxima subgynoecious trait
  • Cucurbita maxima subgynoecious trait molecular marker and primer pair for identification of cucurbita maxima subgynoecious trait
  • Cucurbita maxima subgynoecious trait molecular marker and primer pair for identification of cucurbita maxima subgynoecious trait

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1. Acquisition of SSR Molecular Markers Tightly Linked to Indian Pumpkin Strong Female Gene Cmaf

[0085] In this example, the SSR molecular marker C101 closely linked to the strong female gene Cmaf of Indian squash was obtained. The specific obtaining method includes the following steps:

[0086] (1) Strong female inbred line LC-03 and inbred line 1-14F 2 Generation generation and phenotyping:

[0087] (1) Inbred line 1-14 (male parent, P1) was crossed with strong female inbred line LC-03 (female parent, P2) to obtain hybrid F 1 , F 1 F 2 generation group, F 1 Respectively backcross with P1 and P2 to produce BC 1 P 1 and BC 1 P 2 group.

[0088] (2) Individual plants of different groups are planted in the field, single vines are pruned, managed normally, and the sex type of flowers of each individual plant and each node is investigated and recorded. See Table 1 for statistical analysis results.

[0089] Table 1. Flower traits of the sixth generation...

Embodiment 2

[0111] Embodiment 2, the identification of strong female character of Indian pumpkin

[0112] In this example, the molecular marker C101 obtained in Example 1 was used to identify or assist in the identification of strong female traits of Indian squash.

[0113] 1. Phenotypic identification of Indian pumpkin test materials

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Abstract

The present invention discloses a cucurbita maxima subgynoecious trait molecular marker and a primer pair for identification of cucurbita maxima subgynoecious trait. The cucurbita maxima subgynoecioustrait molecular marker is a DNA molecule obtained by using the primer pair A1 for amplifying on cucurbita maxima genomic DNA as a template, the primer pair A1 comprises a single-stranded DNA respectively named as P1 and P2, the P1 is a single-stranded DNA capable of specifically binding the upstream of the 204th-site of SEQ ID No. 3, and the P2 is a single-stranded DNA capable of specifically binding the downstream of the 219th-site of the SEQ ID No. 3. Experiments show that the primer pair A1 and the cucurbita maxima subgynoecious trait molecular marker for identification or assistant identification of the cucurbita maxima subgynoecious trait can be bed for identification of the condition of the cucurbita maxima subgynoecious trait.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker for the strong female character of Indian squash and a pair of primers for identifying the strong female character of Indian pumpkin. Background technique [0002] my country is the main cultivation country of Indian pumpkins, and its cultivation area and output rank among the top in the world. Especially in recent years, the protected cultivation area of ​​Indian pumpkins in my country has continued to expand, and the market for residents to consume small Indian pumpkins has been gradually cultivated and continuously expanded. The cultivation of Indian pumpkins has It has become an important way to increase farmers' income and adjust agricultural structure. [0003] Indian squash is a typical monoecious crop, with only male or female flowers on the plant, growing at intervals, without the existence of bisexual flowers, and is a typical unisexual floweri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张国裕李海真张帆田佳星贾长才
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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