Nitrogen and phosphorus synergist containing earthworm hydrolysate, as well as preparation method and application of nitrogen and phosphorus synergist

A technology of hydrolyzate and synergist is applied in the agricultural field to achieve the effects of improving soil quality, enhancing soil activity and inhibiting soil-borne diseases

Inactive Publication Date: 2018-06-08
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant research report on the use of earthworm hydrolyzate to improve the utilization rate of nitrogen and phosphorus nutrients

Method used

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  • Nitrogen and phosphorus synergist containing earthworm hydrolysate, as well as preparation method and application of nitrogen and phosphorus synergist
  • Nitrogen and phosphorus synergist containing earthworm hydrolysate, as well as preparation method and application of nitrogen and phosphorus synergist
  • Nitrogen and phosphorus synergist containing earthworm hydrolysate, as well as preparation method and application of nitrogen and phosphorus synergist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Prepare earthworm hydrolyzate. Take 200g of fresh earthworms, wash them 4 times with sterile water, place the washed earthworms in a high-speed tissue masher, and mash them at a speed of 4000r / min for 25 minutes to obtain a homogenate, and mix the homogenate and water at a mass ratio of 1 :3 into the beaker, adjust the pH to 7 with dilute hydrochloric acid with a concentration of 0.02mol / L, then add 500mg of compound enzyme (subtilisin: bromelain=1:2), stir and mix for 4min, then move the beaker into the heat collecting In a magnetic stirrer, adjust the temperature of the stirrer to 42°C, the stirring speed to 230r / min, and the enzymolysis reaction for 4.5h. After the enzymolysis is over, put the enzymolysis solution into a centrifuge and centrifuge at a speed of 4000r / min for 20min. Take the upper layer liquid, that is, the earthworm hydrolyzate, and store it at low temperature for later use.

[0038] (2) Preparation of nitrogen and phosphorus synergists. Mix 18 ...

Embodiment 2

[0048] (1) Prepare earthworm hydrolyzate. Take 150g of fresh earthworms, wash them with sterile water for 5 times, place the washed earthworms in a high-speed tissue masher, and mash them at a speed of 4500r / min for 27 minutes to obtain a homogenate, and mix the homogenate and water at a mass ratio of 1 :2 into the beaker, adjust the pH to 7.5 with dilute hydrochloric acid with a concentration of 0.02mol / L, then add 400mg of compound enzyme (subtilisin: bromelain=1:3), stir and mix for 5min, then move the beaker into the heat collecting In a magnetic stirrer, adjust the temperature of the stirrer to 40°C, and the stirring speed to 220r / min. The enzymolysis reaction is 5h. After the enzymolysis is completed, put the enzymolysis solution in a centrifuge, centrifuge at a speed of 4200r / min for 25min, and take The supernatant liquid, i.e. earthworm hydrolyzate, was stored at low temperature for future use.

[0049] (2) For the preparation of nitrogen and phosphorus synergists, 20...

Embodiment 3

[0060] (1) Prepare earthworm hydrolyzate. Take 100g of fresh earthworms, wash them three times with sterile water, place the washed earthworms in a high-speed tissue masher, and mash them at a speed of 4200r / min for 28 minutes to obtain a homogenate, and mix the homogenate and water at a mass ratio of 1 :2 into the beaker, adjust the pH to 7.5 with dilute hydrochloric acid with a concentration of 0.02mol / L, then add 300mg of compound enzyme (subtilisin: bromelain=1:3), stir and mix for 3min, then move the beaker into the heat collecting In a magnetic stirrer, adjust the temperature of the stirrer to 40°C, and the stirring speed to 200r / min. The enzymolysis reaction is 4h. After the enzymolysis is completed, put the enzymolysis solution into a centrifuge, centrifuge at a speed of 3800r / min for 20min, and take The supernatant is the earthworm hydrolyzate. The earthworm hydrolyzate is vacuum concentrated and dried into powder.

[0061] (2) Preparation of nitrogen and phosphorus...

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Abstract

The invention provides a nitrogen and phosphorus synergist containing an earthworm hydrolysate, as well as a preparation method and application of the nitrogen and phosphorus synergist. The nitrogen and phosphorus synergist containing the earthworm hydrolysate comprises the following components in parts by weight: 15 to 20 parts of the earthworm hydrolysate, 4 to 5 parts of phytase, 15 to 20 partsof citric acid, 20 to 50 parts of potassium fulvic acid and 20 to 25 parts of asparaginic acid. The nitrogen and phosphorus synergist can be prepared into liquid, powder or particles according to requirements. The nitrogen and phosphorus synergist provided by the invention is used on agricultural production, and has the effects of improving the fertilizer efficiency of nitrogen and phosphorus fertilizers, reducing the application amount of nitrogen and phosphorus fertilizers and reducing the non-point source pollution of nitrogen and phosphorus. Greenhouse vegetable planting experiments showthat by using the nitrogen and phosphorus synergist provided by the invention, the greenhouse vegetable yield can be improved, the application of nitrogen and phosphorus fertilizers is reduced, the content of rapidly available phosphorus and rapidly available nitrogen in soil is increased. The soil quality further can be improved, and the soil-borne diseases are inhibited.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a nitrogen and phosphorus synergist containing earthworm hydrolyzate and a preparation method and application thereof. Background technique [0002] In order to pursue high yields of crops such as grain and vegetables, farmers often use large amounts of fertilizers such as nitrogen and phosphorus, so the input of nitrogen and phosphorus in farmland soil is far greater than the demand of crops. If things go on like this, the nutrient content in the soil will continue to accumulate, the absorption and utilization rate of nitrogen and phosphorus by crops will decrease, and the loss of nitrogen and phosphorus will be serious. This will not only cause waste of nutrients, but also bring serious pollution risks to soil, groundwater, and farmland ecological environment. In order to reduce nitrogen and phosphorus pollution and achieve efficient use of nutrients, in additio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/02C05G3/04C05G3/60C05G3/80
CPCC05C11/00C05G3/60C05G3/80C05G5/20C05F1/00C05F11/02C05G3/00
Inventor 井永苹李彦罗加法张英鹏胡浩薄录吉仲子文孙明
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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