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Gene chip for enrichment and extraction of transgenic components and preparation method of gene chip

A technology of genetically modified components and gene chips, which is applied in the fields of biochemical equipment and methods, DNA preparation, and microbial determination/inspection, etc. Effect

Inactive Publication Date: 2018-06-08
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the PCR detection method based on nucleic acid (DNA) requires complex steps such as PCR amplification process, cloning process, and elution process, which require complex and expensive professional technical equipment and professional technicians to complete

Method used

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  • Gene chip for enrichment and extraction of transgenic components and preparation method of gene chip
  • Gene chip for enrichment and extraction of transgenic components and preparation method of gene chip

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preparation example Construction

[0035] The invention provides a method for preparing a gene chip for enrichment and extraction of transgenic components, comprising the following steps:

[0036] S1: Establish a transgene information database; the information in the transgene information database includes: target gene sequence information and general element information; common elements include promoters, terminators and marker genes.

[0037] Among them, if the prepared gene chip is a gene chip enriched and extracted only for the specific transgenic components of a specific genetically modified product, the information in the genetically modified information database includes: the target gene transferred by the specific genetically modified product and / or Common components used in the product;

[0038] If the prepared gene chip is a gene chip for the enrichment and extraction of general-purpose gene components of all genetically modified products, the information in the genetically modified information databa...

Embodiment 1

[0045] This embodiment provides a method for preparing a gene chip for the enrichment and extraction of specific transgenic components only for a specific transgenic product, including the following steps:

[0046] S1: Establish a genetically modified information database; the information of the genetically modified information database includes: the target gene transferred by a specific genetically modified product and the general elements used to obtain the specific genetically modified product; the general elements include promoters, terminators and marker genes.

[0047] S2: Prepare transgenic component capture probes according to the target gene sequence information and general element information, and the transgenic component capture probes are cDNA sequences.

[0048] S3: Design a tether molecule with a length of 25 functional groups, and connect the tether molecule to the 5' end of the probe molecule; wherein, the functional group contains a chimera of cytosine and poly...

Embodiment 2

[0052] This embodiment provides a method for preparing a gene chip for the enrichment and extraction of general gene components for all transgenic products, including the following steps:

[0053] S1: Establish a genetically modified information database; the information of the genetically modified information database includes: all target genes transferred by current genetically modified products and all common elements used by current genetically modified products; common elements include promoters, terminators and marker genes.

[0054] S2: Prepare transgene component capture probes according to the target gene sequence information and general element information, and the transgene component capture probes are oligonucleotides.

[0055] S3: Design a tether molecule with a length of 15 functional groups, and connect the tether molecule to the 5' end of the probe molecule; wherein, the functional group contains polythymidylic acid (Poly T), polyadenylic acid (Poly A A), polyc...

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Abstract

The invention relates to a gene chip for enrichment and extraction of transgenic components and a preparation method of the gene chip. The preparation method comprises the following steps: establishing a transgenic information database including the target gene sequence information and the general element information; then preparing a transgenic component capture probe; designing a connecting armmolecule and connecting the connecting arm molecule with the 5' end of the probe molecule; processing the surface of a solid-phase substrate to prepare an electrode substrate; and fixing the transgenic component capture probe on the surface of the solid-phase substrate through the connecting molecule at the tail end of the connecting arm, so as to obtain the gene chip for enrichment and extractionof the transgenic components. According to the invention, the hybridization efficiency and the hybridization speed of the transgenic components with the extraction chip probe molecule are improved byadopting the combination of the connecting arm and electric field acceleration, and the hybridization time is obviously shortened; and subsequently, the transgenic components bounded by hybridizationare separated from the capture probe through DNA degeneration and rapidly depart from the surface of the extraction chip under the action of a reverse electric field, so that the working efficiency of the chip is obviously improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a gene chip for enrichment and extraction of transgenic components and a preparation method thereof. Background technique [0002] Genetically modified products are products made of animals, plants or microorganisms with specific foreign genes or gene fragments inserted into cell DNA through non-reproductive methods to obtain certain good traits. In recent years, genetically modified products have increasingly become a hot spot of social concern. Therefore, the detection and identification of genetically modified products is becoming more and more important to protect consumers' right to know and right to choose. However, genetically modified products are often not significantly different from non-genetically modified products in terms of appearance and physical and chemical indicators. In this case, without professional testing, it is almost impossible for ordinary consumers to dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B50/06C12Q1/6806C12N15/10
CPCC12N15/1003C12Q1/6806C40B50/06
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