Ammonia-oxidizing bacteria immobilized glass plate and production method thereof

A technology for oxidizing bacteria and glass plates, applied in chemical instruments and methods, water pollutants, water/sludge/sewage treatment, etc., can solve the problems of rising environmental treatment costs, lack of fixed ammonia oxidizing bacteria, etc., and achieve high bacterial activity. , the effect of efficient degradation

Inactive Publication Date: 2018-06-15
光合强化(北京)生物科技有限公司
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AI Technical Summary

Problems solved by technology

Various sewage treatment plants generally use the traditional activated sludge method for biological denitrification in the treatment of domestic sewage and municipal sewage. Due to the rising cost of environmental treatment, immobilized microbial technology has been continuously researched and developed. It will be used in wastewater treatment and even water treatment. Playing an increasingly important role in pollution control, however, there is still a lack of materials for immobilizing ammonia oxidizing bacteria. Therefore, the development of materials for immobilizing ammonia oxidizing bacteria is of great significance for the treatment and restoration of nitrogen-polluted water bodies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039](1) Add glass plates with a length, width and thickness of 10cm, 5cm and 0.5cm respectively into 250mL of NaOH solution with a mass percent concentration of 45%, stir for 5 minutes at 1000r / min, remove the liquid by filtration, and pass through 800mL After washing with deionized water, place it in a drying oven at a temperature of 70°C for 100 minutes to obtain substance A1; polish substance A1 with 200-mesh fine sandpaper to obtain substance A2;

[0040] (2) Add 8.19 grams of potassium hydrogen tartrate to 500 mL of deionized water under stirring condition of 1000 r / min, stir for 3 minutes under the condition of 1000 r / min, then add 3.3 grams of potassium tert-butoxide, shake well to obtain mixed liquid K;

[0041] (3) 2.1 grams of agarose and 1.4 grams of potassium hydrogen phthalate were added to the mixed solution K, and stirred at 1000 r / min for 3 minutes to obtain the mixed solution L;

[0042] (4) Add substance A2 to the mixture L, shake it in a shaker at 40°C for...

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Abstract

The invention discloses an ammonia-oxidizing bacteria immobilized glass plate. A production method of the ammonia-oxidizing bacteria immobilized glass plate comprises the following steps: cleaning a glass plate, and modifying the cleaned glass plate with a mixed solution formed by potassium bitartrate, potassium tert-butoxide, agarose and potassium hydrogen phthalate in order to obtain a substanceA; modifying the substance A with a mixed solution formed by 2-chloro-3-cyanopyrazine, 2-bromo-5-nitropyridine and 4-bromotetrahydropyran in order to obtain a substance B; modifying the substance B with a mixed solution formed by tetrahydrothiopyran-4-one and 1-aminoanthraquinone in order to obtain a substance C; and immobilizing the ammonia-oxidizing bacteria on the substance C to obtain a substance which is the ammonia-oxidizing bacteria immobilized glass plate. The ammonia-oxidizing bacteria immobilized glass plate has the advantages of high strain activity, and efficient degradation of ammonia nitrogen in wastewater.

Description

technical field [0001] The invention belongs to the field of research and development and preparation of functional materials for environmental pollution control technology, and in particular relates to a glass plate for immobilizing ammonia oxidizing bacteria and a preparation method thereof. Background technique [0002] Immobilized microorganism technology is a biotechnology developed from immobilized enzyme technology in biochemical industry in the 1960s. It uses physical or chemical methods to fix free microbial cells into particles through self-agglomeration or in a specific cushion material. It remains active and can be used repeatedly. In the late 1970s, with the increasingly serious water pollution, this technology was applied to the biological treatment of industrial wastewater due to its unique advantages. Compared with the traditional suspension biological treatment method, immobilized microorganism technology has the ability to improve the microbial Cell concen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/16
CPCC02F3/34C02F2101/16
Inventor 不公告发明人
Owner 光合强化(北京)生物科技有限公司
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