Method for removing bisphenol A by utilizing crude enzyme solution of michelia maudiae leaves
A crude enzyme liquid, deep mountain technology, applied in chemical instruments and methods, special compound water treatment, water pollutants, etc., can solve the problems of harsh catalyst conditions, reduced application scenarios, long time consuming for microorganisms, etc., to improve comprehensive economic benefits, The effect of abundant resources and high catalytic efficiency
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Embodiment 1
[0031] Example 1 The effect of different enzyme concentrations on the degradation of BPA in the crude enzyme solution of leaves of Michelia miracosa.
[0032] Each reaction system is placed in 3 numbered reagent bottles, a total of 3 reagent bottles, each reagent bottle is added with phosphoric acid-citric acid buffer solution with a pH value of 7, and then BPA is added to make the final concentration 0.2mmol / L , Then add 100U, 200U, 300U of enzyme solutions containing different enzyme activities, the reaction system is 20mL in total, take 1mL sample and add 1mL methanol as a blank control, and add a clean magnetic stirring stone into it. Place the reagent bottles in a constant temperature water bath and stir at room temperature, add hydrogen peroxide solution to react for 180min according to the molar concentration ratio of hydrogen peroxide and BPA of 1:2, and react for 1min, 3min, 5min, 15min, 45min, 90min, 120min 180min, take 1mL of the reaction mixture, add 1mL of methano...
Embodiment 2
[0033] Example 2 The effect of different temperature conditions on the degradation of BPA by the crude enzyme solution from the leaves of Michelia miracosa.
[0034] Take 5 reagent bottles, add an appropriate amount of phosphoric acid-citric acid buffer solution with a pH value of 7 to each reagent bottle, then add BPA solution to make the final concentration 0.2mmol / L, and then add a certain amount of enzyme solution to make the enzyme activity unit When it reaches 15U / mL, keep the total volume at 20mL (the insufficient part is supplemented with phosphoric acid-citric acid buffer solution with a pH value of 7), mix well, take 1mL of the mixed solution, add 1mL of methanol as a blank control, and add magnetic force to the mixed solution. stir bar. Place the reagent bottle in a constant temperature magnetic stirring water bath with a water temperature of 20°C, 30°C, 40°C, 50°C, and 60°C for stirring, and add hydrogen peroxide solution according to the molar concentration ratio ...
Embodiment 3
[0035] Example 3 The effect of pH on the degradation of BPA by the crude enzyme solution from the leaves of Michelia miracosa.
[0036] Each reaction system is placed in 9 numbered reagent bottles, a total of 9 reagent bottles, use acetic acid-sodium acetate, phosphoric acid-citric acid, borax buffer to control the pH of the reaction system to be 3, 4, 5, 6, 7, 8, 9, 10, 11, then add BPA solution to make the final concentration 0.2mmol / L, then add enzyme solution to make the enzyme activity unit reach 15U / mL, keep the total volume at 20mL For the buffer solution of 7), take 1mL sample, add 1mL methanol as a blank control, and add a clean magnetic stirring stone to it. Place the reagent bottles in a magnetic stirrer to react at room temperature, add hydrogen peroxide to start the reaction according to the molar concentration ratio of hydrogen peroxide and BPA of 1:2, and react for 1min, 3min, 5min, 15min, 45min, 90min, Take 1mL of the reaction mixture at 120min and 180min, res...
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