Compound in sabia parviflora and preparation method and application thereof

A compound and supernatant technology, applied in the field of medicine, can solve the problems of unclear active substances and unknown active ingredients in liver diseases, and achieve the effects of reducing apoptosis rate, simple operation and increasing MDA content

Active Publication Date: 2018-06-15
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Qingfengteng and its related varieties are mostly used clinically for diseases related to the liver. However, the main active ingredients in Qingfengteng are unknown at present, and the active substances of its liver diseases are still unclear

Method used

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  • Compound in sabia parviflora and preparation method and application thereof
  • Compound in sabia parviflora and preparation method and application thereof
  • Compound in sabia parviflora and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of the new compound in embodiment 1, the little flower Qingfengteng

[0030] (1) Take the dried herb, add 12 times the concentration of 70% ethanol, reflux and extract three times for 3 hours, 2 hours, and 2 hours respectively, filter, combine the extracts, and concentrate under reduced pressure until there is no alcohol smell , get the extract;

[0031] (2) Take the extract of step (1) and add ethanol to make the alcohol content 20%, remove the insoluble matter by centrifugation, and obtain the supernatant.

[0032] (3) Get the supernatant that step (2) obtains and absorb through HP-20 type macroporous resin column (medicinal material: resin=1:2 adsorption), first use 30% ethanol-water eluting 5 column volumes, obtain stream Fraction 1; Fraction 2 was obtained by eluting with 50% ethanol-water for another 5 column volumes, concentrating and drying. Fraction 2 was subjected to 200-300 mesh silica gel column chromatography, and the mixed solution...

Embodiment 2

[0040] Embodiment 2, drug effect experimental research

[0041] 1. Experimental materials and animals

[0042] 1. Drugs and reagents

[0043] Human normal liver cell line L-02 cells (Shanghai Huiying Biotechnology Co., Ltd.), fetal bovine serum (Hangzhou Sijiqing Bioengineering Materials Co., Ltd.), RPMI Medium 1640 medium (Solarbio Company), oleic acid (OA, O7501) , palmitic acid (PA, P9767) were purchased from SIGMA Company, biochemical detection kit (Nanjing Jiancheng Bioengineering Research Institute Co., Ltd.), trypsin-EDTA digestion solution (Solarbio Company).

[0044] 2. Experimental equipment

[0045] Inverted microscope (Olympus-ckx41), biological safety cabinet (Heal Force), AL204 electronic analytical balance (Mettler Toledo Instruments (Shanghai) Co., Ltd.), MTV-100 multi-tube vortex mixer (Hangzhou Aosheng Instruments Co., Ltd.).

[0046] 3. Test drugs and treatment methods

[0047] Take the compound prepared in Example 1 and add DMSO to dilute it to a soluti...

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PUM

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Abstract

The invention belongs to the technical filed of medicine, and discloses a compound in sabia parviflora and a preparation method and an application thereof. The compound is 2-methyl-2-hydroxymethyl-5,6-dihydroxy-7-(3-methyl-3-hydroxy-1-butenyl)-1H-indene-1-ketone-3-O-glucoside. The preparation method of the compound is simple, and obtain the pure compound through separation. The experiment shows that the compound can obviously reduce the hepatocyte triacylglycerol content and caspase3 activity due to aliphatic acid, increases the MDA content, reduces hepatocyte apoptosis rate, and can be usedfor preventing non-alcoholic fatty liver damage. The invention provides the application of the compound in preparation of medicines for treating non-alcoholic fatty liver damage.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a compound in Qingfengteng and its preparation method and application, especially the application in the preparation of medicine for treating non-alcoholic fatty liver injury. Background technique [0002] Nonalcoholic fatty liver disease (NAFLD), also known as nonalcoholic fatty liver disease and pseudoalcoholic liver disease, is a clinicopathological syndrome with liver histological changes similar to alcoholic liver disease but without a history of excessive drinking. Changes with the progression of the disease showed simple fatty liver, steatohepatitis, fatty liver fibrosis and liver cirrhosis. With the global prevalence of obesity and related metabolic syndrome, non-alcoholic fatty liver disease has become an important cause of chronic liver disease in developed countries such as Europe and the United States and in wealthy areas of my country. The prevalence of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08A61K31/704A61P1/16
CPCC07H1/08C07H15/203
Inventor 李志峰冯育林王琦欧阳辉李俊何明珍杨世林樊东辉
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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