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Method for increasing tomato seed germination rate under drought stress and observation device

A drought stress and observation device technology, which is applied in the field of plant physiology research, can solve the problems of inability to effectively and intuitively observe the germination form of seeds, limited seed growth space, poor air permeability of the device, etc. And the effect of flexible repetition and sufficient growth space

Active Publication Date: 2018-06-29
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The existing seed germination device is relatively complicated, simple but with a large workload, and the poor air permeability of the device causes the seeds to become moldy, increases the experimental error, and limits the growth space of the seed after germination to a certain extent, and cannot effectively and intuitively observe the shape of the seed germination

Method used

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  • Method for increasing tomato seed germination rate under drought stress and observation device
  • Method for increasing tomato seed germination rate under drought stress and observation device
  • Method for increasing tomato seed germination rate under drought stress and observation device

Examples

Experimental program
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Effect test

Embodiment 1

[0048] The present embodiment provides a method for improving the germination rate of tomato seeds under drought stress, comprising the steps of:

[0049] (1) Drought tolerance concentration screening

[0050] PEG-6000 was used to simulate drought stress. The concentration of the treatment solution was set to be 0 (CK), 5%, 10%, 15%, and 20% PEG-6000, respectively. Clean and assemble the seed germination observation device and dry it for later use. Pick cherry tomato seeds that are plump and uniform in size, after disinfection, soak the seeds in the dark at room temperature with 20ml of each treatment solution for 24 hours, then wash with distilled water three times and blot dry, put the radicle germination position down and place it in the seed germination tank of the seed germination observation device. Place two grains in the net position, place 40 grains repeatedly in this way, and inject half of its volume of treatment solution 1 into the glass tank of the seed germinat...

Embodiment 2

[0070] The present embodiment provides tomato seed germination observation device, as attached Figure 3-4 As shown, its structure includes: a seed germination tank 1, a screen 2 is placed inside the seed germination tank 1, a perforated scale tube 4 is movably connected to the bottom of the seed germination tank 1, and a filter paper 3 is placed in the perforated scale tube 4; a perforated scale tube 4 Put it on the hose rack 6 and fix it. The perforated scale tube 4 is placed in the glass tank 5; the partition plate 7 is placed in the glass tank 5;

[0071] Further, the shape of the seed germination tank 1 is funnel-shaped, so that a large area is in contact with air to ensure the air permeability of the device.

[0072] Further, the filter paper 3 is rolled into an arc shape and placed in the perforated scale tube 4, occupying two-thirds of the circumference of the perforated scale tube 4, and the scale is exposed.

[0073] Furthermore, both the glass tank 5 and the plasti...

Embodiment 3

[0079] The present embodiment provides a kind of observation method of tomato seed germination, as attached Figure 3-5 shown, including the following steps:

[0080] (1) Clean the seed germination tank 1, the screen 2, the perforated scale tube 4, the glass tank 5, the hose rack 6 and the glass partition 7, and dry them for later use.

[0081] (2) Assemble each component according to the brief description of the manual and the attached drawings. First roll the filter paper 3 into an arc shape and place it in the graduated tube with holes 4, occupying two-thirds of the circumference of the graduated tube with holes 4, exposing the scale. Spread the screen 2 on the top of the perforated scale tube 4, and then fix the seed germination tank 1 on the top of the perforated scale tube 4; then place this assembly on the hose holder 6, and the hose holder 6 is inserted into the glass tank 5 Fix the upper side, put the glass tank 5 into the rubber sleeve 10, and finally use the nut 8...

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Abstract

The invention provides a method for increasing a tomato seed germination rate under drought stress. According to the invention, 8-Br-cGMP with appropriate concentration is used for processing tomato seeds, and the germination rate of the tomato seeds under drought stress can be increased. In addition, the invention also provides a seed germination observation device by simulating natural light induction condition, and comparison and form observation of the seeds in a tomato seed germination test are realized. The used 8-Br-cGMP has membrane permeability and high utilization rate, the low-concentration membrane 8-Br-cGMP can obviously increase the tomato seed germination rate under drought stress. The used device has the advantages of simple structure, simple operation and strong repeatability, and can be used for real-time observation of different seed germination conditions, in addition, the processing and repetition of the device are more flexible.

Description

technical field [0001] The invention relates to the field of plant physiology research, in particular to a method for increasing the germination rate of tomato seeds under drought stress and a matching observation device. Background technique [0002] In recent years, more and more attention has been paid to the study of biological hormones and signaling molecules on plant seed germination, growth and development under stress, especially in signal transduction and biological stress resistance. Recognized six biological hormones: auxin (IAA), gibberellin (GA), cytokinin (CTK), abscisic acid (ABA), ethylene (ETH) and brassinosterol (BR), and the signaling molecule salicyl Acid (SA), nitric oxide (NO), carbon monoxide (CO), etc. have been successively used in the stress resistance research of plants. A large number of experimental results show that trace amounts of plant hormones and small molecule signaling substances are key factors regulating seed dormancy and germination, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 魏小红宿梅飞韩厅赵颖张小芳朱雪妹刘放
Owner GANSU AGRI UNIV
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