Nanoparticles with AIAE (aggregation-induced absorption enhancement) phenomenon as well as synthesis and application of nanoparticles

Abstract

The invention discloses nanoparticles with an AIAE (aggregation-induced absorption enhancement) phenomenon as well as synthesis and an application of the nanoparticles and relates to nanoparticles. The nanoparticles with the AIAE phenomenon comprise inner cores and outer layers, wherein the inner cores are croconaine analogues, the outer layers are protein particles, the inner cores are loaded ininner cavities of the outer layers, and composite nanostructures with the croconaine analogues as the inner cores and the protein particles as the outer layers are formed. Hepatitis B core protein particles are diluted and added to a depolymerization buffer solution to be stirred; dye is uniformly stirred in DMSO, and croconaine analogue dye is obtained; the croconaine analogue dye is added to anobtained mixture and stirred, and a mixed liquid is obtained; the mixed liquid is added to a dialysis bag, dialysis is performed in an assembling buffer solution 1, and a magnetic stirring reaction isperformed; the mixed liquid is added to the dialysis bag, dialysis is performed in an assembling buffer solution 2, and a magnetic stirring reaction is performed to obtain medicine-carrying protein nanoparticles; the obtained medicine-carrying protein nanoparticles are subjected to <125>I labeling, and the nanoparticles with the AIAE phenomenon are obtained.

Description

technical field [0001] The invention relates to a nano particle, in particular to a nano particle with an aggregation-induced light absorption enhancement phenomenon, a synthesis method and an application thereof. Background technique [0002] Early diagnosis of tumors can help doctors identify tumor tissues earlier and reduce patients' pain. Photoacoustic imaging technology combines the high contrast of optics and the penetration depth of ultrasound. Through targeted probes, it can specifically identify tumor tissues and normal tissues, helping doctors to quickly and accurately monitor cancerous tissues and reduce damage to normal tissues. Therefore, Two-zone photoacoustic probes targeting tumor tissue imaging have attracted widespread attention. Photoacoustic imaging technology is a new type of non-destructive real-time imaging method, which combines the high contrast of optical imaging and the high penetration depth of ultrasonic imaging. Characteristics, metabolic func...

AI Technical Summary

Problems solved by technology

Long-wavelength near-infrared light has the characteristics of deep penetration in the body, less scattering, and low fluorescence interference. However, most organic small molecule dyes are insoluble in water, and are easy to aggregate on the surface of biological macromolecules through hydrophobic interactions, making the small dye molecules localized. As the concentration increases, fluorescence quenching occurs, which greatly limits further biological applications

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