An electrochemical polymer molecularly imprinted biomimetic electrochemical probe, its preparation and application
A molecular imprinting and molecularly imprinting membrane technology, which is applied in the fields of electrochemical variables of materials, material analysis by electromagnetic means, scientific instruments, etc., can solve the problems that tetrabromobisphenol A cannot be easily detected online, and achieves good practical prospects. Strong practicability and good stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0051] A method for preparing an electrochemical polymer molecularly imprinted biomimetic electrochemical probe provided by the present invention comprises the following steps:
[0052] (1) The glassy carbon electrode is placed in a mixed solution containing functional monomer dopamine, template molecule tetrabromobisphenol A and 0.01 moles per liter of phosphate buffer solution with a pH of 6.0 to 8.0, and the embedded electrode is prepared by cyclic voltammetry. The polydopamine film of tetrabromobisphenol A template molecule; wherein the concentration ratio of dopamine and template molecule tetrabromobisphenol A is 5:1~20:1; the concentration of template molecule tetrabromobisphenol A is 0.25~1.0 milligrams moles per liter; the buffer solution is preferably 0.01 moles per liter of phosphate buffer solution with a pH of 7.5.
[0053] (2) The eluent is used to elute the tetrabromobisphenol A template molecule under stirring conditions for 0.5 to 2 hours; the scanning potentia...
Embodiment 1
[0060] Preparation of electrochemical polymer biomimetic molecularly imprinted membrane (molecular imprinting is a chemical method that simulates the combination of antigen-antibody in nature, so it is called biomimetic):
[0061] Place the glassy carbon electrode polished to a mirror surface in 0.01 moles per liter of phosphate buffer containing 0.5 millimoles of dopamine and 0.5 millimoles per liter of the template molecule tetrabromobisphenol A, and scan the potential range by cyclic voltammetry: -0.5 ~0.5 volts, the scanning speed is 75 millivolts per second, and the number of scanning cycles is 10 cycles to prepare a polydopamine film embedded with tetrabromobisphenol A template molecules. Then methanol: acetic acid (4:1) solution was used as the eluent, the tetrabromobisphenol A template molecule was eluted under stirring for 1 hour, and dried naturally. Then, a small amount of residual tetrabromobisphenol A was removed by constant potential oxidation at 1 volt to obtain...
Embodiment 2
[0069] The commonly used three-electrode system is adopted, the electrochemical polymer molecularly imprinted bionic electrochemical sensor probe is used as the working electrode, the saturated calomel electrode is used as the reference electrode, and the platinum column electrode is used as the counter electrode. The experimental data is collected and analyzed by connecting the electrochemical workstation and the computer. Record. In the case of sufficient stirring, differential pulse voltammetry was used to detect tetrabromobisphenol A (TBBPA) and the response signals after adding high concentration phenol (phenol), p-chlorophenol (Chlorophenol) and bisphenol A (BPA). The detection potential range is 0.3-1.0 volts, and the medium is 5.00 ml, 0.01 moles per liter of pH 6.0 phosphate buffer solution. After enrichment with stirring for 3 minutes, the peak intensities were determined by differential pulse voltammetry as 40 NM tetrabromobisphenol A and after adding high concentra...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
| length | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More