Grass carp feed with health-caring functions based on probiotic resistance to stress

Abstract

The invention discloses a grass carp feed with health-caring functions based on probiotic resistance to stress. The grass carp feed with health-caring functions based on probiotic resistance to stressis prepared by the following steps: S1, selecting pseudoalteromonas nigrifaciens, lactic acid bacillus, bacillus subtilis and bacillus bifidus of the same number of bacteria colonies, respectively inoculating the selected pseudoalteromonas nigrifaciens, lactic acid bacillus, bacillus subtilis and bacillus bifidus into primary culture media, and carrying out primary proliferation culture for 20-25hours so as to obtain primary proliferation culture liquid; S2, mixing the primary proliferation culture liquid obtained in the S1, inoculating the mixture into culture liquid in a fermentation tank,and carrying out secondary proliferation culture for 30-35 hours so as to obtain feed fermentation broth; and S3, adding a feed base material into the feed fermentation broth obtained in the S2, carrying out fermentation for 15-20 hours, filtering the fermentation broth so as to obtain a solid material and aqueous fermentation liquid, drying the solid material so as to obtained a dried material ready for using, performing spray-drying on the aqueous fermentation liquid by using a high-speed centrifugal spray-drying machine so as to obtain a fine bacterium powder material, and uniformly mixingthe fine bacterium powder material with the dried material so as to obtain a final-product feed.

Description

technical field [0001] The invention relates to the field of preparation of probiotic fish feed, in particular to a grass carp feed based on probiotics for anti-stress and health care. Background technique [0002] At present, the search for alternatives to antibiotics has become an inevitable trend in the development of the aquaculture industry. The development direction of feed additives has shifted to green feed additives that are harmless to human and animal health, including probiotics, enzyme preparations, organic acids, and organic trace elements. . Among them, probiotics, as a class of safe and effective live bacteria preparations, have become an important additive for breeding, especially fish farming. On the other hand, developing ecological control technology and reducing the use of antibiotics is the theme of healthy aquaculture in the 21st century. Probiotics are an important technical pillar of healthy farming. With the development of the breeding industry, ...

AI Technical Summary

Problems solved by technology

[0003] Based on the beneficial effects of probiotics in the aquaculture industry, a series of patent documents on the use of probiotics in aquaculture have been reported successively. For example, the Chinese patent number is: CN. Its preparation method", however, the growth rate of fish schools can be improved to a certain extent through this technical solution, but the survival rate and weight gain rate of fish schools are not significantly improved. In view of the shortcomings of existing technologies, there is no real-time and effective way so far solve