Hemoglobin hemolytic agent and application thereof

A technology of hemoglobin and hemolyzing agent, applied in the field of blood cell analysis, can solve the problems of inaccurate measurement results of hemoglobin, achieve the effect of wide clinical use, prevent false increase of hemoglobin, and ensure accuracy

Active Publication Date: 2018-07-24
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a hemolytic agen

Method used

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  • Hemoglobin hemolytic agent and application thereof
  • Hemoglobin hemolytic agent and application thereof
  • Hemoglobin hemolytic agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0063] Prepare the solution according to the following scheme:

[0064]

[0065] Mix sodium dodecylsulfonate, Tween 20, dodecyldimethyl betaine, and sodium hydroxide according to the above ratio, add deionized water to 1L, stir at room temperature until completely dissolved, and filter through a 0.2μm filter membrane Filter and test on a blood cell analyzer, the blank value meets the requirements, and the results of hemoglobin content are shown in Table 3.

Embodiment example 2

[0067] Prepare the solution according to the following scheme:

[0068]

[0069] Mix sodium lauryl sulfate, triton 100, octadecyl dimethyl betaine, and sodium hydroxide according to the above ratio, add deionized water to 1L, stir at room temperature until completely dissolved, and filter through a 0.2 μm filter membrane Filter, test on a hematology analyzer, the blank value meets the requirements, and determine the hemoglobin content. The results are shown in Table 2.

Embodiment example 3

[0071] Prepare the solution according to the following scheme:

[0072]

[0073] Mix sodium lauryl sulfate, AEO-9, octadecyldimethyl betaine, and sodium hydroxide according to the above ratio, add deionized water to 1L, stir at room temperature until completely dissolved, and filter with a 0.2 μm filter membrane. Filter and test on a hematology analyzer, the blank value meets the requirements, and the results of hemoglobin content are shown in Table 3.

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PUM

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Abstract

The invention discloses a hemoglobin hemolytic agent and the use thereof, the hemoglobin hemolytic agent comprises a) at least one sufficient amount of a nonionic surfactant for dissolving red blood cells, b) at least one sufficient amount of an amphoteric surfactant for dissolving chyle particles, and c) at least one anionic surfactant that binds to hemoglobin. The pH of the hemoglobin hemolyticagent is between 5 and 8. After being mixed with a blood sample, the hemoglobin hemolytic agent can be used for measurement of hemoglobin content. Since the surfactant which reacts with the chyle particles is added, the interference of sample difference is eliminated, the accuracy of the measurement of the hemoglobin content is ensured, a clinical use range is wider, and a false hemoglobin increase can be prevented.

Description

technical field [0001] The invention relates to the field of blood cell analysis, and mainly relates to a hemolytic agent for hemoglobin and an application thereof. The hemolytic agent for hemoglobin can accurately measure the hemoglobin in chyle blood. Background technique [0002] The determination of hemoglobin is an important indicator of the oxygen-carrying capacity of the blood, and chyle blood is "blood with oil slicks" or "lipemia", and the plasma is milky white or turbid. Clinically, the proportion of chyle blood samples is as high as 1%. Because chyloblood contains a large number of chylogranules, which increases the turbidity of plasma, it will cause falsely high HGB measurement results and bring troubles to clinical diagnosis. Accurate measurement of hemoglobin in blood is an important basis for the diagnosis of related diseases. After the sample is diluted, a hemolytic agent is added to dissolve red blood cells and chylogranules, release hemoglobin, and hemoglo...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5005
Inventor 余晓尘郑剑通李春晖张勇亮
Owner SHENZHEN DYMIND BIOTECH
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