Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for removing hydroquinone in α-arbutin conversion solution

A technology of arbutin and transformation liquid, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problem of unsuitable separation, large pollution, and loss of a large amount of arbutin α-arbutin transformation liquid to remove impurities And other issues

Active Publication Date: 2020-10-27
ANHUI HUAHENG BIOTECH +3
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the fermentation production of α-arbutin, a small amount of hydroquinone with poor stability usually remains, which affects the safety of α-arbutin as a cosmetic additive. Therefore, how to effectively remove the α-arbutin in the fermentation production The hydroquinone produced is a technical problem to be solved urgently in this area
[0003] In the prior art, CN102001917A and CN102001760A respectively disclose a method for concentrating and separating hydroquinone and a method for decolorizing and concentrating and separating hydroquinone, but both methods have the problem of losing a large amount of arbutin and Not suitable for impurity removal of α-arbutin conversion solution in this application
In addition, CN103483401B discloses a method for extracting and separating hydroquinone, although the method can efficiently extract hydroquinone in α-arbutin fermentation broth at a lower cost, but the method steps are cumbersome, and There is a problem of solvent residue, it is not suitable for large-scale system separation, and it is difficult to apply to industrial production
It can be seen that the current existing methods for isolating hydroquinone from fermented liquid have shortcomings such as high cost, large pollution, and low efficiency to varying degrees, and need to be improved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for removing hydroquinone in α-arbutin conversion solution
  • Method for removing hydroquinone in α-arbutin conversion solution
  • Method for removing hydroquinone in α-arbutin conversion solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the removal of hydroquinone in α-arbutin conversion solution

[0025] (1) Escherichia coli is collected after seed culture and fermentation culture;

[0026] (2) to 2m 3 Inject 0.7m into the conversion tank 3 water, then add 340kg of sucrose, 13.2kg of hydroquinone, after the dissolution is complete, dilute to 1m 3 , then add 0.1m to the conversion tank 3 Add 5L of surfactant to the bacteria, react at 30°C for 3 hours, the conversion is complete, the residual hydroquinone in the product is 0.56g / L, the content of α-arbutin is 30.5g / L, and the molar conversion rate of hydroquinone is 93.4%;

[0027] (3) The product was incubated at 60°C for 1 hour, and after microfiltration treatment through a ceramic membrane with a pore size of 100 nm, the permeate was then ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 2000, and the ultrafiltration permeate was collected;

[0028] (4) At room temperature and natural pH, the colle...

Embodiment 2

[0031] Embodiment 2: the removal of hydroquinone in α-arbutin conversion liquid

[0032] (1) After the Bacillus subtilis is cultivated by seed and fermented, the thalline is collected;

[0033] (2) to 2m 3 Inject 0.7m into the conversion tank 3 water, then add 340kg of sucrose, 13.2kg of hydroquinone, after the dissolution is complete, dilute to 1m 3 , then add 0.1m to the conversion tank 3 Add 5L of surfactant to the bacteria, react at 30°C for 3 hours, the conversion is complete, the residual hydroquinone in the product is 0.56g / L, the content of α-arbutin is 30.5g / L, and the molar conversion rate of hydroquinone is 93.4%;

[0034] (3) The product is incubated at 65° C. for 1 hour, and after microfiltration treatment through a ceramic membrane with a pore size of 100 nm, the permeate is then ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 2000, and the ultrafiltration permeate is collected;

[0035] (4) At room temperature and natural...

Embodiment 3

[0037] Embodiment 3: the removal of hydroquinone in α-arbutin conversion solution

[0038] (1) After Xanthomonas campestris is cultivated by seeds and fermented, the thalline is collected;

[0039] (2) to 2m3 Inject 0.7m into the conversion tank 3 water, then add 340kg of sucrose, 13.2kg of hydroquinone, after the dissolution is complete, dilute to 1m 3 , then add 0.1m to the conversion tank 3 Add 5L of surfactant to the bacteria, react at 30°C for 3 hours, the conversion is complete, the residual hydroquinone in the product is 0.56g / L, the content of α-arbutin is 30.5g / L, and the molar conversion rate of hydroquinone is 93.4%;

[0040] (3) The product is incubated at 70° C. for 1 hour, and after microfiltration treatment through a ceramic membrane with a pore size of 100 nm, the permeate is then ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 2000, and the ultrafiltration permeate is collected;

[0041] (4) Under normal temperature and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biochemical engineering, in particular to a removal method of hydroquinone in Alpha-arbutin conversion liquid. The method concretely comprises the stepsthat after saccharose, the hydroquinone, a surfactant and thalli react, the Alpha-arbutin conversion liquid is obtained; filtering is performed; resin is used for performing adsorption treatment on filtering liquid; then, resin permeation liquid is collected; an Alpha-arbutin solution in which the hydroquinone is completely removed is obtained. The method provided by the invention has the advantages that the hydroquinone in the Alpha-arbutin conversion liquid can be thoroughly removed; the cost is low; no pollution exists; the efficiency is high, and the like.

Description

technical field [0001] The invention relates to the technical field of biochemical industry, in particular to a method for removing hydroquinone in α-arbutin conversion liquid. Background technique [0002] α-arbutin is an epimer of β-arbutin, and its chemical name is 4-hydroxyphenyl-α-D-glucopyranoside. Studies have shown that α-arbutin is more stable than β-arbutin, and has better anti-inflammatory, repairing and whitening effects. It is often used as a whitening agent in high-end skin care products and cosmetics. In the fermentation production of α-arbutin, a small amount of hydroquinone with poor stability usually remains, which affects the safety of α-arbutin as a cosmetic additive. Therefore, how to effectively remove the α-arbutin in the fermentation production The hydroquinone that produces is a technical problem that needs to be solved urgently in this area. [0003] In the prior art, CN102001917A and CN102001760A respectively disclose a method for concentrating a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/06
CPCC07H1/06C07H15/203
Inventor 刘树蓬韩成秀郭恒华张冬竹田宋魁章晖
Owner ANHUI HUAHENG BIOTECH