A fluorescent oxygen probe and its preparation method and application

Abstract

The invention provides a fluorescent oxygen probe, which comprises polymer microspheres copolymerized with a reference probe compound and fluorescent oxygen uniformly dispersed in the polymer microspheres copolymerized with a reference probe compound. Probe compound, the present invention also provides a creative method for preparing the fluorescent oxygen probe, by copolymerizing the reference probe compound with the polymer monomer to prepare microspheres dispersed with the fluorescent oxygen probe compound, the The microspheres can be used as fluorescent oxygen probes. The fluorescent oxygen probes prepared by the present invention have a self-reference effect, which avoids the problem of recalibrating the fluorescent probes when replacing detection equipment in traditional fluorescent detection. The detection environment is not demanding, and it can be used for the detection of dissolved oxygen concentration in dynamic biological environments such as water bodies containing microorganisms. Compared with the detection results of traditional electrochemical detection equipment, the error of the detection results is ≤5%.

Description

technical field [0001] The invention relates to the field of fluorescence detection, in particular to a fluorescent oxygen probe and its preparation method and application. Background technique [0002] Oxygen is an indispensable substance in life and is closely related to human life. How to quickly and accurately detect oxygen concentration is of great significance in fisheries, food safety, environmental testing, and biomedicine. [0003] So far, there are many methods for detecting oxygen concentration. Specific detection methods such as chemical titration detection, electrochemical detection, fluorescence detection, etc., the traditional chemical titration method has the highest detection accuracy, but the traditional chemical titration method needs to be equipped with a standard concentration solution, the steps are cumbersome, time-consuming, and complicated to operate. It is only suitable for some precise tests. The electrochemical detection method obtains the oxygen ...

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Problems solved by technology

[0003] So far, there are many methods for detecting oxygen concentration. Specific detection methods such as chemical titration detection, electrochemical detection, fluorescence detection, etc., the traditional chemical titration method has the highest detection accuracy, but the traditional chemical titration method needs to be equipped with a standard concentration solution, the steps are cumbersome, time-consuming, and complicated to operate. It is only suitable for some precise tests. The electrochemical detection method obtains the oxygen concentration by detecting the reduction potential of oxygen at the anode. It has a good application in the detection of dissolved oxygen, but its The current is easily affected by many factors such as electromagnetic interference, and the detection results have certain accidental errors. Moreover, limited by the size of the equipment, this method cannot be applied to some small areas. Fluorescence detection is a new detection method. Due to its It has many advantages such as high efficiency, good selectivity, convenient detection, and short response time, and is favored by the fields of analytical chemistry, bioanalytical chemistry, and environmental science.

[0004] Fluorescence detection methods usually require the participation of fluorescent probes. When the fluorescent probe is combined with the sample to be tested, its fluorescence intensity or fluorescence emission peak needs to change significantly, and the change can be detected by the fluorescence detector. However, the existing technology The use of fluorescence detection method to detect oxygen concentration usually faces a problem, that is, most of the fluorescent substances that can be used as fluorescent probes to detect oxygen are relatively poor in stability and prone to photobleaching, resulting in a decrease in fluorescence efficiency and large deviations in the detection results. , and because the fluorescence intensity units of various types of fluorescence spectrometers are not uniform, the fluorescent probes can usually only be used with specific types of fluorescence spectrometers. The standard curve can only be used after re-making the standard curve. The above shortcomings limit the further promotion of the fluorescence detection method.

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