Hydrogel 3D printing material and preparation method thereof
A 3D printing and hydrogel technology, applied in the direction of additive processing, etc., can solve the problems of sol shaping difficulties, toxic cells, and easily damaged structures, and achieve non-toxic cells and tissues, good biocompatibility, and promote The effect of cell growth
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Embodiment 1
[0016] Embodiment 1: the preparation method of hydrogel 3D printing material
[0017] (1) Preparation of gel A: at room temperature, add 12 parts of amino hyaluronic acid to 25 parts of water and stir to fully dissolve, then drop in 8 parts of cellulose and stir thoroughly, and finally add 5 parts of cationic chitin and stir to mix well , then vortex the resulting mixture for 30 seconds, and place it in a water bath at 37°C for 2 minutes to obtain gel A;
[0018] (2) Preparation of gel B: Stir and mix 15 parts of alkaline gelatin and 35 parts of alginic acid at room temperature, then vortex the resulting mixture for 30 seconds, and place it in a water bath at 37°C for 2 minutes. That is B gel.
Embodiment 2
[0019] Embodiment 2: the preparation method of hydrogel 3D printing material
[0020] (1) Preparation of gel A: at room temperature, add 10 parts of aminated hyaluronic acid to 27 parts of water and stir to fully dissolve, then drop in 10 parts of cellulose and stir well, and finally add 3 parts of cationic chitin and stir to mix well , then vortex the resulting mixture for 50 seconds, and place it in a 35°C water bath for 3 minutes to obtain A gel;
[0021] (2) Preparation of gel B: Stir and mix 18 parts of alkaline gelatin and 32 parts of alginic acid at room temperature, then vortex the resulting mixture for 50 seconds, and place it in a water bath at 35°C for 3 minutes. That is B gel.
Embodiment 3
[0022] Embodiment 3: the preparation method of hydrogel 3D printing material
[0023] (1) Preparation of gel A: at room temperature, add 13 parts of amino hyaluronic acid to 25 parts of water and stir to fully dissolve, then drop in 6 parts of cellulose and stir thoroughly, and finally add 6 parts of cationic chitin and stir to mix well , then vortex the obtained mixture for 40 seconds, and place it in a water bath at 37°C for 2 minutes to obtain gel A;
[0024] (2) Preparation of gel B: Stir and mix 18 parts of alkaline gelatin and 32 parts of alginic acid at room temperature, then vortex the resulting mixture for 40 seconds, and place it in a water bath at 37°C for 2 minutes. That is B gel.
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