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SNP genetic markers for bone density traits in sows

A genetic marker and bone mineral density technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of expensive equipment, troublesome operation, rare livestock skeleton research, etc., and achieve high sensitivity and specificity. good effect

Active Publication Date: 2020-12-29
HUAZHONG AGRI UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that bone density is an important indicator in the study of animal bone strength, but due to the limitations of various production conditions, people's research on livestock bones is very rare
[0004] Bone density, that is, the content of bone mineral per unit volume, the unit is g / cm 3 , common bone density measurement methods include radiation absorption method, dual-energy X-ray measurement method, quantitative computed tomography technology and axial ultrasonic transmission technology (Cao Jian, application evaluation of bone density (BMD) measurement[J]. Frontiers of Medicine ,2013(2).), among which dual-energy X-ray measurement and quantitative computed tomography are the most widely used, but the area bone density measured by dual-energy X-ray measurement is affected by the geometric volume of the object, while quantitative computed tomography Scanning technology can measure animal bone density more accurately, but the equipment is expensive (Liu Jun, a comparative study of dual-energy X-ray absorptiometry and quantitative CT measurement of bone density [D], Central South University, 2005), and the operation is very troublesome (For large livestock, it needs to be performed after anesthesia), which greatly limits its application in animal bone density research

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  • SNP genetic markers for bone density traits in sows
  • SNP genetic markers for bone density traits in sows
  • SNP genetic markers for bone density traits in sows

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Genotyping Detection

[0025] (1) Using the kit method to extract ear tissue DNA from bone density-related populations

[0026] 1) Cut the ear-like tissues of bone density-related populations (this population includes Danish (Denmark) Large White and Changbai, which are conventional varieties, provided by Guangxi Yangxiang Co., Ltd.) with ophthalmic scissors until they are paste, add 200ulGA ( kit), and vortexed to mix; then placed in a 56°C water bath for overnight digestion.

[0027] 2) Add 200ul buffer GB (included with the kit) to the digested tissue sample, mix thoroughly by inversion, and place at 70°C for 10 minutes, the solution should become clear, and centrifuge briefly to remove water droplets on the tube wall.

[0028] 3) Add 200ul of absolute ethanol, shake and mix well for 15 seconds, at this time flocculent precipitation may appear, briefly centrifuge to remove water droplets on the tube wall.

[0029] 4) Add the solution and flocculent preci...

Embodiment 2

[0036] Example 2: Application of the genetic markers screened by the present invention and its typing method in the correlation analysis of bone density traits of pig limbs and hooves (1) correlation analysis of genetic marker typing results and bone density traits of limbs and hooves

[0037] The experimental pigs used for the correlation detection and analysis of genotype and bone density traits of limbs and hooves come from purebred sow groups bred by Guangxi Yangxiang Co., Ltd., including Large White pigs and Landrace pigs (conventional breeds). The DNA used for genotyping is extracted from the ear samples of purebred Large White pigs and Landrace pigs ("Purebred Large White and Landrace" referred to in the text of the instructions and tables are referred to as "pigs" for short). Using the method based on the single-marker association regression model, using the gender of the individual as a fixed effect, the GWAS analysis was performed using the GenABEL software package in...

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Abstract

The invention belongs to the technical field of molecular marker screening of animals and particularly relates to an SNP (Single Nucleotide Polymorphism) genetic marker for sow limb coffin bone density property. The molecular marker is cloned from gene segments with a login number of ALGA0082045. The genes are subjected to typing screening by virtue of a gene chip technology so as to obtain a molecular marker relating to sow limb coffin bone density. A nucleotide sequence of the marker is shown as SEQ ID NO:1, a C / T allelic variant exists at the base at the 51st site of the sequence so as to cause polymorphism of the sequence SEQ ID NO:1. When the nucleotide at the 51st site of the sequence is C, the limb coffin bone of the sow has higher bone density. The invention further discloses a screening method for the molecular marker related to sow limb coffin bone density property and application thereof in correlation analysis.

Description

technical field [0001] The invention relates to the technical field of preparation of pig genetic markers, in particular to SNP genetic markers for sow limb and hoof bone density traits. The genetic markers described in the invention can be used to predict the bone density traits of pig limbs and hooves. Background technique [0002] With the development of science and technology, the pig breeding industry has made significant breakthroughs in genetic breeding, nutritional regulation, and disease prevention and control. The lean meat rate, feed-to-meat ratio, and growth rate of commercial pigs have all been greatly improved. However, with intensive farming comes the increasingly prominent problem of limbs and feet. For intensive production, the range of activities of pigs is limited. In addition, the design of pens (slatted floors) has exacerbated the occurrence of pigs' limbs and feet. It has been reported that 20-50% of all gilts passed the performance test were culled fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杜小勇冯帅飞刘小磊尹立林马官军叶灯灯唐振双赵书红李新云
Owner HUAZHONG AGRI UNIV
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