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Device and method for fluids separation by density gradient

A technology of density gradient separation and density, which is applied in the field of kits, special centrifuge tubes/containers, and devices for performing the method, and can solve problems such as difficulty in implementation, cumbersome procedures, and high maintenance costs

Inactive Publication Date: 2018-08-03
SPARK TECH SP ZOO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, all of these procedures are cumbersome, difficult to perform, introduce the possibility of random human error, and additionally require highly qualified personnel, which introduces high maintenance costs, reduces the repeatability of the procedure and makes it impossible Perform large-scale separations

Method used

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  • Device and method for fluids separation by density gradient
  • Device and method for fluids separation by density gradient
  • Device and method for fluids separation by density gradient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Such as figure 1 As shown, in a first embodiment of the invention, the means 6 of a centrifuge container in the form of a centrifuge tube consists of a flat disc 7 that fits tightly to the inner wall of the tube divider and another disc 8 and attached to the disc 8 The full vertical divider 11 of the disc 7 and 8 constitutes the device divider. In this embodiment of the invention, the device is placed within a centrifuge container 1, which is a 0.23 inch diameter centrifuge tube. In this embodiment, the device 6 is made of plastic, but it could also be made of other materials. Such as Figure 12 As shown, the device 6 can be placed in another container which can be fitted to the centrifuge container 1 , in which case the device is outside the centrifuge container 1 .

[0048] In this embodiment, the inner wall of the centrifuge container 1 is at the same time the guide 12 and the wall of the centrifuge container becomes thicker, the inner diameter of the centrifuge c...

Embodiment 2

[0058] Figure 13 and 13a Another embodiment of the invention is shown in which the device 6 has a partition 7 which does not have an upper chamber but allows connection down to a container with a separation medium or liquid (e.g. , test tubes, sachets, bags) form the upper divider. Subsequently, the divider is fitted with a guide 12 in the form of an elongated cylinder attached to the divider 7 and located at a distance from the hole 4 . This allows fluid to flow from the upper chamber 16 through the tubes and then along the guides to the lower chamber 3 through holes in the partition. In this embodiment, an elongated cylinder forms the guide 12 and is of such a length that the test material spreads smoothly over the surface of the centrifugation medium used in the gradient separation method without causing significant disturbance to the separation medium.

Embodiment 3

[0060] Figure 14 and 14a Another embodiment of the invention is shown in which the insert 6 has a spacer 7 fitted with guides 12 in the form of eight elongated rollers anchored to the spacer 7 and located at a distance from the hole 4 at such a distance, this allows liquid to flow from the upper chamber to the lower chamber 3 along the guides through the holes in the partition. In this embodiment, the length of the guide 12 of the elongated roller is such that the test material spreads smoothly over the surface of the centrifugation medium used in the gradient separation method and it does not cause significant disturbance to the separation medium.

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PUM

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Abstract

The device for a centrifugation container, particularly to a tube, for separation of liquid fractions having a desired density range, in particular invention applies to biological and / or liquids forming suspensions, characterized by the device having a partition (7) that separates the interior of the container (1) into at least two chambers in a vertical arrangement - an upper chamber (2) and alower chamber (3), and the device having the partition (7) has an aperture (4) which can be lined up with the guide (12), on which liquids, in particular fluid sample, can flow down from upper chamber(3) to lower chamber (4), of the container (1) for centrifugation. The invention also include the method for separating out a fraction having the desired density range from the sample containing fractions of different density with use of the device according to the invention.

Description

technical field [0001] The invention relates to a device, a container with the device and a method for fluid separation by means of density gradient centrifugation. At the same time, the invention relates to a kit for carrying out the method. Specifically, the present invention is used to separate body fluids such as animal blood, human blood, for further analysis like clinical diagnosis or research. The invention relates to the field of containers used in laboratories, in particular to special centrifuge tubes / containers. Another use of the invention relates to the field associated with the testing or analysis of materials, in particular the analysis of liquid biological materials, such as blood, by determining their chemical, physical or biological properties. Background technique [0002] The collection, purification, separation into various fractions, and / or preservation of fluid samples, including blood, play an important role in medical diagnosis as well as in clinic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5021B01L2200/16B01L2300/0609B01L2300/087B01L2400/0633B01L2400/0644
Inventor M·G·阿达姆斯基P·谷曼
Owner SPARK TECH SP ZOO