SSR (simple sequence repeat) primer and method for watermelon hybrid variety purity identification

Abstract

The invention discloses an SSR (simple sequence repeat) primer for watermelon hybrid seed purity identification. The primer comprises an upstream primer CISSR09643-F and a downstream primer CISSR09643-R of a primer CISSR09643, the nucleotide sequence of the upstream primer CISSR09643-F is as shown in SEQ ID NO. 1, and the nucleotide sequence of the downstream primer CISSR09643-R is as shown in SEQID NO. 2. The invention further discloses a method for watermelon hybrid seed purity identification. The method is used for identifying the purity of hybrid seeds of watermelon varieties such as Bolin, Ande, Tianfu No. two and yellow phoenix. According to the primer and the method, the purity of the hybrid seeds of the four watermelon varieties of Bolin, Ande, Tianfu No. two and yellow phoenix can be effectively identified only by simply extracting a watermelon leaf genome DNA (deoxyribonucleic acid) and then performing PCR (polymerase chain reaction) amplification and polyacrylamide gel electrophoresis, a traditional hybrid seed purity identification method can be replaced, identification time is greatly shortened, identification cost is saved, identification accuracy is improved, and high commercial application values are achieved.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to an SSR primer and a method for identifying the purity of watermelon hybrid varieties. Background technique [0002] Watermelon is a cross-pollinated plant that is widely cultivated throughout my country. In recent years, watermelon hybrid varieties have been rapidly popularized and promoted in my country, and the cultivated area has expanded year by year. It has become one of the important crops to increase farmers' income and promote rural economic development. [0003] Utilizing heterosis and cultivating a generation of hybrid watermelon varieties is the main method of watermelon breeding at present. In the process of watermelon hybrid seed production, due to reasons such as untimely and incomplete artificial castration, false hybrids often appear, resulting in a decline in the genetic purity of seeds and causing huge economic losses to production. Water...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the defects that current watermelon hybrid seed purity identification mostly relies on field phenotype observation method, which is limited by seasons and takes a long time for identification. The first purpose of the present invention is to provide a kind of SSR for watermelon hybrid seed purity identification Primers, the primers can produce specific markers in the corresponding parents, and the specificity is strong; the second purpose of the present invention is to provide a method for identifying the purity of watermelon hybrid seeds, which is used to identify the seeds of Bolin, Ander, and Tianfu. The purity of 4 kinds of watermelon hybrid seeds of No. 1 and Huangfenghuang, this method can quickly, accurately and effectively identify the purity of 4 kinds of watermelon hybrid seeds by using the above SSR primers;

Images