Method for quick propagation by means of hemp stem tip tissue culture

A tissue culture and shoot tip technology, which is applied in the field of cannabis shoot tip tissue culture and rapid propagation, can solve problems such as affecting the tightness of the experimental cycle.

Active Publication Date: 2018-08-17
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hypocotyls of the aseptic seedlings produced by inoculating the medium after seed disinfection are used as explants. It takes more than 100 days from seed inoculation to rooting to obtain new plants, which affects the tightness of the experimental cycle.

Method used

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  • Method for quick propagation by means of hemp stem tip tissue culture
  • Method for quick propagation by means of hemp stem tip tissue culture
  • Method for quick propagation by means of hemp stem tip tissue culture

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Experimental program
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Effect test

specific Embodiment approach 1

[0034] A method for rapid propagation of cannabis stem tip tissue culture, comprising the steps of:

[0035] Step 1. Seed disinfection: select mature, plump and shiny hemp seeds, wash them with washing liquid first, then rinse them with tap water, then soak them in 75% ethanol in an ultra-clean bench, then rinse them with sterile water, and then use Soaking in mercuric chloride solution with a concentration of 0.1%, then rinsing with sterile water until the water body is clear, drying the surface moisture of the seeds with sterile filter paper, to obtain sterilized hemp seeds;

[0036] Step 2. Germination culture: Inoculate the hemp seeds sterilized in step 1 into the following germination medium, MS medium, 30g / L sucrose, 8g / L agar, pH 5.8, culture temperature 23-25°C, light intensity 2300lux , cultivated under 16h light and 8h dark conditions every day, and cultivated for 10 days to obtain the stem tip of aseptic cannabis seedlings;

[0037] Step 3, regeneration culture: In...

specific Embodiment approach 2

[0047] A method for rapid propagation of cannabis stem tip tissue culture, comprising the steps of:

[0048] Step 1. Seed disinfection: select mature, plump and shiny hemp seeds, wash them with washing liquid first, then rinse them with tap water, then soak them in 75% ethanol in an ultra-clean bench, then rinse them with sterile water, and then use Soaking in mercuric chloride solution with a concentration of 0.1%, then rinsing with sterile water until the water body is clear, drying the surface moisture of the seeds with sterile filter paper, to obtain sterilized hemp seeds;

[0049] Step 2. Germination culture: inoculate the hemp seeds sterilized in step 1 into the following germination medium, MS medium, sucrose 25g / L, agar 7g / L, pH 5.7, culture temperature 23-25°C, light intensity 1800lux , cultivated under 16h light and 8h dark conditions every day, and cultivated for 10 days to obtain the stem tip of aseptic cannabis seedlings;

[0050] Step 3, regeneration culture: In...

specific Embodiment approach 3

[0059] A method for rapid propagation of cannabis stem tip tissue culture, comprising the steps of:

[0060] Step 1. Seed disinfection: select mature, plump and shiny hemp seeds, wash them with washing liquid first, then rinse them with tap water, then soak them in 75% ethanol in an ultra-clean bench, then rinse them with sterile water, and then use Soaking in mercuric chloride solution with a concentration of 0.1%, then rinsing with sterile water until the water body is clear, drying the surface moisture of the seeds with sterile filter paper, to obtain sterilized hemp seeds;

[0061] Step 2, germination culture: inoculate the hemp seeds sterilized in step 1 in the following germination medium, MS medium, sucrose 30g / L, agar 8g / L, pH5.9, culture temperature is 25°C, light intensity is 2300lux, Cultivate under the conditions of 16 hours of light and 8 hours of darkness every day for 8 days to obtain the stem tips of aseptic cannabis seedlings;

[0062] Step 3, regeneration cu...

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Abstract

The invention discloses a method for quick propagation by means of hemp stem tip tissue culture, and belongs to the technical field of plant artificial cultivation. The method includes seed disinfection, germination culture, regeneration culture and rooting culture. Hormone-containing MS (Murashige and Skoog) culture media for the regeneration culture comprise sucrose with the concentration of 25-30 g / L and agar with the concentration of 7-8 g / L, the pH (potential of hydrogen) is 5.7-5.9, the culture temperatures are 23-25 DEG C, the illumination intensity is 1800-2300 lux, and the regeneration culture is carried out under 16h-illumination and 8h-dark conditions on a daily basis for 20-30 days. Hormone-containing MS culture media or hormone-containing 1 / 2 MS culture media for the rooting culture comprise sucrose with the concentration of 30 g / L and agar with the concentration of 8 g / L, the pH is 5.8, the culture temperatures are 23-25 DEG C, the illumination intensity is 2300 lux, andthe rooting culture is carried out under 16h-illumiantion and 8h-dark conditions on a daily basis for 20 days. The method has the advantages that regenerated plants can be quickly propagated by meansof stem tip tissue culture, and the method is short in experimental period.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of plants; in particular, it relates to a rapid propagation method of hemp stem tip tissue culture. Background technique [0002] Cannabis (Cannabis Sativa L.) is an annual herbaceous plant of the genus Cannabis in the family Cannabinaceae. Hemp is one of the oldest and oldest cultivated crops in the world and in China. Hemp is full of treasures and can be extended from agricultural planting to textiles, clothing, paper, military supplies, chemicals, new building materials, bio-energy, food health care, medicine, feed Wait for more than a dozen industrial chains. [0003] Because marijuana contains a hallucinogenic active ingredient-tetrahydrocannabinol (THC), which is easily used by criminals to illegally produce drugs and cause social harm, many countries have also listed marijuana as a prohibited crop, causing many Precious germplasm resources are lost, and research on hemp lag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姜颖孙宇峰潘冬梅韩喜财夏尊民宋淑敏肖宇韩承伟王晓楠赵越曹焜魏联会高媛
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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