A kind of rapid propagation method of hemp stem tip tissue culture

A technology of tissue culture and stem tip, which is applied in the field of rapid propagation of cannabis stem tip tissue culture, can solve the problems affecting the tightness of the experimental cycle, etc., and achieve the effect of improving the survival rate of transplanting, accelerating the improvement process, and stabilizing excellent traits

Active Publication Date: 2021-05-25
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hypocotyls of the aseptic seedlings produced by inoculating the medium after seed disinfection are used as explants. It takes more than 100 days from seed inoculation to rooting to obtain new plants, which affects the tightness of the experimental cycle.

Method used

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  • A kind of rapid propagation method of hemp stem tip tissue culture
  • A kind of rapid propagation method of hemp stem tip tissue culture
  • A kind of rapid propagation method of hemp stem tip tissue culture

Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0035] A method for rapid propagation of cannabis stem tip tissue culture, comprising the steps of:

[0036] Step 1. Seed disinfection: select mature, plump and shiny hemp seeds, wash them with washing liquid first, then rinse them with tap water, then soak them in 75% ethanol in an ultra-clean bench, then rinse them with sterile water, and then use Soaking in mercuric chloride solution with a concentration of 0.1%, then rinsing with sterile water until the water body is clear, drying the surface moisture of the seeds with sterile filter paper, to obtain sterilized hemp seeds;

[0037] Step 2. Germination culture: Inoculate the hemp seeds sterilized in step 1 into the following germination medium, MS medium, 30g / L sucrose, 8g / L agar, pH 5.8, culture temperature 23-25°C, light intensity 2300lux , cultivated under 16h light and 8h dark conditions every day, and cultivated for 10 days to obtain the stem tip of aseptic cannabis seedlings;

[0038] Step 3, regeneration culture: In...

specific Embodiment approach 2

[0049] A method for rapid propagation of cannabis stem tip tissue culture, comprising the steps of:

[0050] Step 1. Seed disinfection: select mature, plump and shiny hemp seeds, wash them with washing liquid first, then rinse them with tap water, then soak them in 75% ethanol in an ultra-clean bench, then rinse them with sterile water, and then use Soaking in mercuric chloride solution with a concentration of 0.1%, then rinsing with sterile water until the water body is clear, drying the surface moisture of the seeds with sterile filter paper, to obtain sterilized hemp seeds;

[0051] Step 2. Germination culture: inoculate the hemp seeds sterilized in step 1 into the following germination medium, MS medium, sucrose 25g / L, agar 7g / L, pH 5.7, culture temperature 23-25°C, light intensity 1800lux , cultivated under 16h light and 8h dark conditions every day, and cultivated for 10 days to obtain the stem tip of aseptic cannabis seedlings;

[0052] Step 3, regeneration culture: In...

specific Embodiment approach 3

[0062] A method for rapid propagation of cannabis stem tip tissue culture, comprising the steps of:

[0063] Step 1. Seed disinfection: select mature, plump and shiny hemp seeds, wash them with washing liquid first, then rinse them with tap water, then soak them in 75% ethanol in an ultra-clean bench, then rinse them with sterile water, and then use Soaking in mercuric chloride solution with a concentration of 0.1%, then rinsing with sterile water until the water body is clear, drying the surface moisture of the seeds with sterile filter paper, to obtain sterilized hemp seeds;

[0064] Step 2, germination culture: inoculate the hemp seeds sterilized in step 1 in the following germination medium, MS medium, sucrose 30g / L, agar 8g / L, pH5.9, culture temperature is 25°C, light intensity is 2300lux, Cultivate under the conditions of 16 hours of light and 8 hours of darkness every day for 8 days to obtain the stem tips of aseptic cannabis seedlings;

[0065] Step 3, regeneration cu...

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Abstract

The invention relates to a rapid propagation method of hemp stem tip tissue culture, which belongs to the technical field of artificial cultivation of plants. The invention includes seed disinfection, germination culture, regeneration culture, rooting culture, regeneration culture MS medium containing hormone, sucrose 25-30g / L, agar 7-8g / L, pH 5.7-5.9, culture temperature 23-25°C , the light intensity is 1800-2300lux, cultured under 16h light and 8h dark conditions every day, and the culture days are 20-30 days; rooting culture contains hormone-containing MS medium or hormone-containing 1 / 2MS medium, sucrose 30g / L, agar 8g / L, pH 5.8, culture temperature 23-25°C, light intensity 2300 lux, culture under 16h light and 8h dark conditions every day, and culture time is 20 days. The invention utilizes shoot tip tissue culture to rapidly propagate to obtain regenerated plants, and the experiment period is short.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of plants; in particular, it relates to a rapid propagation method of hemp stem tip tissue culture. Background technique [0002] Cannabis (Cannabis Sativa L.) is an annual herbaceous plant of the genus Cannabis in the family Cannabinaceae. Hemp is one of the oldest and oldest cultivated crops in the world and in China. Hemp is full of treasures and can be extended from agricultural planting to textiles, clothing, paper, military supplies, chemicals, new building materials, bio-energy, food health care, medicine, feed Wait for more than a dozen industrial chains. [0003] Because marijuana contains a hallucinogenic active ingredient-tetrahydrocannabinol (THC), which is easily used by criminals to illegally produce drugs and cause social harm, many countries have also listed marijuana as a prohibited crop, causing many Precious germplasm resources are lost, and research on hemp lag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姜颖孙宇峰潘冬梅韩喜财夏尊民宋淑敏肖宇韩承伟王晓楠赵越曹焜魏联会高媛
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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