A traditional Chinese medicine composition for chemosensitization of breast cancer and its preparation method
A breast cancer and composition technology, applied in the field of traditional Chinese medicine compositions and preparations for breast cancer chemotherapy sensitization, can solve the problems that do not meet the modernization and internationalization of traditional Chinese medicine, the medicinal substances and curative effects are not clear, and the quality control indicators are not strict. and other problems, to achieve the effect of inhibiting the growth of tumor cells, conducive to prevention and treatment, and small side effects
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[0054] (2) Preparation and determination of the test solution, place 0.1g of the Chinese medicine composition weighed in a 100mL conical flask, accurately add 20mL of methanol, ultrasonically extract for 60min, let it stand to cool, methanol makes up for weight loss, and take the supernatant An appropriate amount was passed through a 0.22 μm filter membrane to prepare the test solution, and 10 μL of the test solution was injected into the chromatographic system for determination.
[0055] (3) Preparation and determination of the reference substance solution, dissolve p-coumaric acid, calycosin glucoside, liquiritin, glycyrrhizic acid and curcumol reference substance with methanol to be the reference substance stock solution of appropriate concentration, then dilute with methanol respectively For different concentration gradients, pass through a 0.22 μm filter membrane to prepare a reference solution, inject 10 μL of the reference solution into the chromatographic system for mea...
Embodiment 1
[0067] Embodiment 1 decoction
[0068] Prescription: 15g decoction pieces of Hedyotis diffusa, 15g decoction pieces of Zezhu, 15g decoction pieces of Astragalus membranaceus, 15g decoction pieces of licorice.
[0069] Preparation method: Weigh each decoction piece, add 1200-1800mL of water, soak for half an hour, decoct for 1 hour, filter, continue decocting the dregs for 2 times, mix the filtered medicinal liquid from 3 times of decoction, decoct and concentrate to 1000mL.
[0070] Usage and dosage: Drink 1000mL (equivalent to 60g of the original medicine) daily, three times a day.
Embodiment 2
[0071] Embodiment 2 Granules
[0072] Prescription: 30g decoction pieces of Hedyotis diffusa, 30g decoction pieces of Zezhu, 30g decoction pieces of Astragalus membranaceus, 30g decoction pieces of licorice.
[0073]Preparation method: Weigh each raw material, pulverize, pass through No. 1 sieve, use 12 times, 10 times and 8 times the amount of 95% ethanol to reflux extract three times, each time for 1 hour, filter, the filtrate stands still, and take the supernatant , combined the supernatants extracted three times, concentrated under reduced pressure to form a paste, evaporated ethanol to obtain the raw material extract of the Chinese medicine compound preparation, added suitable excipients, flavoring agents and other common excipients to make granules, and took it as a single dose Divide into granules to obtain granules.
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