Arabidopsis thaliana drought-resistant agent, preparation method and use method
A drought-resistant agent, the technology of Arabidopsis thaliana, applied in application, microbial treatment, organic fertilizer, etc., can solve the problems of poor safety and degradation of drought-resistant agents, and no water absorption, so as to improve soil compaction, increase absorption rate, The effect of increasing relative water content of leaves
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0028] Based on the same inventive concept, the present invention also provides a method for preparing an Arabidopsis drought-resistant agent, comprising the following steps:
[0029] S1, preparation of fermented corn steep liquor
[0030] Mix sterilized corn kernels, sterile water, Bacillus subtilis and Bifidobacteria in proportions of 1000:10 to 15:3:5 by weight, ferment at 20-30°C for 3 days, sterilize, and grind into corn slurry, then add water equivalent to 50 times the quality of corn steep liquor, stir, filter, and the collected filtrate is fermented corn steep liquor, and the filter residue can be used as organic fertilizer or Arabidopsis culture substrate for Arabidopsis cultivation;
[0031] S2, preparing fermented soybean milk
[0032] Mix sterilized soybeans, sterile water, and Bacillus subtilis inoculum at a weight ratio of 1000:10 to 15:3, ferment at 20-30°C for 5 days, sterilize, grind into soybean milk, and then add 50 times the mass of soybean milk water, st...
Embodiment 1
[0038] An Arabidopsis drought resistance agent is prepared from the following raw material components in parts by weight: 60 parts of fermented corn steep liquor, 40 parts of fermented soybean milk, 10 parts of potassium fulvic acid, 3 parts of gibberellin and 2 parts of soluble starch. The preparation method of the Bacillus subtilis bacterial agent is as follows: the Bacillus subtilis seed liquid is inoculated in a fermenter according to the volume fraction of 5% inoculum, and cultivated at 30° C. with a stirring speed of 200 r / min for 24 hours, and the obtained fermented liquid is passed through 80 mesh The rice bran is adsorbed and dried to obtain the Bacillus subtilis inoculum; the preparation method of the bifidobacteria inoculum and the Bacillus subtilis inoculum is identical; 10 cfu / g. Described Arabidopsis drought resistance agent is prepared according to the following steps:
[0039] S1, preparation of fermented corn steep liquor
[0040] Mix sterilized corn kernels...
Embodiment 2
[0047] An Arabidopsis drought resistance agent is prepared from the following raw material components in parts by weight: 50 parts of fermented corn steep liquor, 45 parts of fermented soybean milk, 15 parts of potassium fulvic acid, 5 parts of gibberellin and 3 parts of soluble starch. The preparation method of the Bacillus subtilis bacterial agent is as follows: the Bacillus subtilis seed liquid is inoculated in a fermenter according to the volume fraction of 3% inoculum, cultivated at a temperature of 35°C and a stirring speed of 200r / min for 18h, and the obtained fermented liquid is subjected to 80 The objective rice bran is adsorbed and dried to obtain Bacillus subtilis inoculum; the preparation method of bifidobacterium inoculum and Bacillus subtilis inoculum is the same; the number of live bacteria in both Bacillus subtilis inoculum and Bifidobacterium inoculum is 10 10 cfu / g. Described Arabidopsis drought resistance agent is prepared according to the following steps: ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More