Nucleic acid containing photosensitive unit and preparation method thereof
A photosensitive unit, nucleic acid technology, applied in the field of nucleic acid modification, can solve the problems of inability to meet scientific research and commercial needs, cumbersome synthesis steps, difficult separation and purification, etc., and achieves good experimental operability, simplified operation steps, and simplified modification steps.
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[0036] An embodiment of the present invention also provides a method for preparing a nucleic acid containing a photosensitive unit, comprising the following steps:
[0037] Step 1: Preparation of phosphoramidite ester: including using 5-hydroxy-2-nitrobenzyl alcohol as starting material, selectively protecting its benzyl alcohol hydroxyl group by 4,4'-dimethoxytriphenyl chloride, After etherification reaction to obtain 5-hydroxy-2-nitrobenzyl-(4,4'-dimethoxytriphenyl) ether, 2-nitrobenzyl-(4 ,4'-dimethoxytriphenyl) ether-5-(2-O-cyanoethyl-N,N-diisopropylphosphoramidite);
[0038] Step 2: preparing nucleic acid containing photosensitive unit: dissolving the phosphoramidite in anhydrous acetonitrile as an end group modification reagent and synthesizing nucleic acid containing photosensitive unit to obtain nucleic acid containing photosensitive unit.
[0039]In an optional embodiment, the synthesized product in the second step is dispersed in ammonia water, sealed and heated, ce...
Embodiment 1
[0060] A method for preparing a nucleic acid containing a photosensitive unit, comprising the following steps:
[0061] Step 1: Preparation of 5-hydroxy-2-nitrobenzyl-(4,4'-dimethoxytriphenyl) ether: Dissolve 1.7 g of 5-hydroxy-2-nitrobenzyl alcohol in 100 ml of 4.0 g of 4,4'-dimethoxytriphenylchloromethane dissolved in 30 ml of anhydrous pyridine was added dropwise to the water pyridine under stirring in an ice bath, and the reaction solution was stirred at 0°C after the dropwise addition was completed. After 1 hour, return to room temperature and stir overnight; after the reaction is completed, the organic phase is rotary evaporated, the crude product is dissolved in 30 ml of ethyl acetate, washed twice with 50 ml of water, and the organic phase is dried with anhydrous sodium sulfate; filtered; After the organic solvent was removed by rotary evaporation, the obtained dark yellow oily liquid was dissolved in 5 ml of ethyl acetate, and added dropwise to 100 ml of n-hexane cool...
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