Method for extracting and purifying polysaccharide from okra and method for identifying monosaccharide ingredient

An identification method and okra technology are applied in the fields of biological extraction and monosaccharide component identification, which can solve the problems of being unsuitable for large-scale promotion and high cost, and achieve the effects of improving extraction rate, improving polysaccharide purity and simple process.

Inactive Publication Date: 2018-09-04
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although some okra polysaccharide extraction methods have been reported in the literature, the cost is too high to be suitable for large-scale promotion

Method used

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  • Method for extracting and purifying polysaccharide from okra and method for identifying monosaccharide ingredient
  • Method for extracting and purifying polysaccharide from okra and method for identifying monosaccharide ingredient
  • Method for extracting and purifying polysaccharide from okra and method for identifying monosaccharide ingredient

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Embodiment Construction

[0017] The present invention will be described in detail below in conjunction with specific embodiment: a kind of method of extracting and purifying polysaccharide from okra according to the present invention, this method comprises the following steps:

[0018] (a) Pretreatment: Take fresh tender okra fruit, sieve to remove the attachments on the surface of okra, cut the okra into cubes, remove the okra seeds by filtration, add 4 to 8 times to the crushed okra weight of water, use a homogenizer or a colloid mill to make the crushed okra into a homogenate;

[0019] (b) Extraction: the okra homogenate is filtered by filtration, and the filter cake and filtrate are collected respectively.

[0020] (c) Enzymolysis: add the protease of 0.1~2.0% okra weight in the filtrate, add the calcium chloride of 0.05%-1% raw material weight, adjust pH to be 8-9, stir in 30~50 ℃ scope 6~ 10h;

[0021] (d) Precipitation treatment: adjust the pH of the enzymatic solution to 3.0-5.5, add sulfate...

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Abstract

The invention relates to a method for extracting and purifying polysaccharide from okra and a method for identifying a monosaccharide ingredient. The method comprises the following steps: a) taking fresh tender okra fruits, and making the pulverized okra top into a homogenate or a colloid grinder to a homogenized state; (2) filtering the okra homogenate by employing a filtering method, and respectively collecting a filter cake and a filtrate; c) adding 0.1-2.0% of protease according to weight of okra in the filtrate, adding 0.05-1% of calcium chloride according to weight of raw materials, adjusting the pH value to 8-9, and stirring the materials for 6-10 h at the temperature of 30-50 DEG C, d) adjusting the pH value of enzymatic hydrolysis liquid to 3.0-5.5 and adding a sulfuric acid salting solution, uniformly stirring the materials, performing standing and processing the filtrate by using a centrifugal process, and collecting the filtrate; and e) performing ultrafiltration treatmenton the obtained filtrate, collecting trapped fluid, and performing spray drying to obtain the okra polysaccharide powder. The method has the characteristics of simple process of polysaccharide extraction and monosaccharide identification, and can increase the okra polysaccharide recovery rate.

Description

technical field [0001] The invention relates to a method for extracting and purifying polysaccharides from okra and a method for identifying monosaccharide components, and belongs to the technical field of biological extraction and monosaccharide component identification. Background technique [0002] Okra is an annual herb of Malvaceae (Malvaceae) okra, and okra originates in Africa, Egypt and other places, and is currently planted in large areas in Europe and Africa. As a vegetable, medicine, and flower dual-purpose plant, its uses are quite extensive. In recent years, okra is mainly cultivated by picking tender pods as vegetables, and can also be used to prepare solid beverages, substitute teas, and polysaccharide products. The functional properties of okra are mainly manifested in its high nutritional value and remarkable health care and therapeutic effects. From a nutritional point of view, okra is rich in protein, fat, carbohydrates, vitamins, minerals and dietary fib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/00C08B37/0003
Inventor 孙坤来
Owner ZHEJIANG OCEAN UNIV
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