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Establishing method of chicken H9 subtype avian influenza artificial infection model

A construction method and avian influenza technology, applied in the field of livestock and poultry disease model construction, can solve the problems of no symptoms, unfavorable correct evaluation of the efficacy of avian influenza prevention and treatment drugs, restriction of the research and development of avian influenza prevention and treatment drugs, etc. Appropriate rate and obvious effect of onset symptoms

Inactive Publication Date: 2018-09-14
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0003] In previous studies, the following difficulties existed in the construction of the pathogenesis model of avian influenza: when the low pathogenic H9 subtype AIV was used to challenge SPF chickens, the symptoms of the disease were very mild, and no obvious symptoms were clinically manifested; When the pathogenic H5 subtype AIV is challenged, due to the strong virulence, almost 100% of the infected chickens die, and the clinical trials of H5 subtype highly pathogenic avian influenza are strictly controlled by the country
The above two situations are not conducive to making a correct evaluation of the efficacy of avian influenza control drugs, which seriously restricts the research and development of avian influenza control drugs

Method used

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  • Establishing method of chicken H9 subtype avian influenza artificial infection model
  • Establishing method of chicken H9 subtype avian influenza artificial infection model

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Experimental program
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Embodiment 1

[0015] 1. Materials and methods

[0016] 1.1. Virus strain: H9 subtype AIV strain, EID 50 for EID 50 =10 -6.83 / 0.1mL; IBV M41 strain, EID 50 1.0×10 -6.5 / 0.1mL; Avian Escherichia coli c84008 strain.

[0017] 1.2. Experimental animals: 130-day-old SPF young laying hens

[0018] 1.3. Test method

[0019] (1) Virus challenge: On November 16, 2017, 8 130-day-old SPF young laying hens were raised in SPF chicken feeding isolators. Starting from 2 days before infection, the feeding temperature was set at 15°C. Each chicken Firstly instill 0.5mL of H9AIV stock solution through the nose, and then instill 0.5mL of the stock solution of IBV M41 strain after dripping all the chickens, and then each chicken instills 0.5mL of the fresh broth culture of Escherichia coli c84008 strain; 30min;

[0020] (2) Observation of clinical symptoms and pathological changes: After inoculation, observe the clinical symptoms and death conditions of the flock of chickens every day, and make records...

Embodiment 2

[0043] 1. Materials and methods

[0044] 1.1. Virus strain: H9 subtype AIV strain, EID 50 for EID 50 =10 -6.83 / 0.1mL; IBV M41 strain, EID 50 1.0×10 -6.5 / 0.1mL; Avian Escherichia coli c84008 strain.

[0045] 1.2. Test animals: 140-day-old SPF laying hens

[0046] 1.3. Test method

[0047](1) Virus challenge: On December 5, 2017, 10 140-day-old SPF young laying hens were raised in SPF chicken rearing isolators, and the feeding temperature was controlled at 10°C from 2 days before infection. Intranasal H9AIV stock solution 0.5mL, after dripping all chickens, then instill 0.3mL of IBV M41 strain stock solution, and then each chicken intranasally drips 0.3mL of fresh broth culture of Escherichia coli c84008 strain; the interval infection time of each stock solution is 25min ;

[0048] (2) Test observation: After the virus challenge, observe the clinical symptoms and death of the chickens every day, and make a record; during the test, measure the weight gain rate, blood sa...

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Abstract

The invention relates to the technical field of livestock and poultry pathogenesis model establishment, in particular to an establishing method of a chicken H9 subtype avian influenza artificial infection model. In the method, newly-developed SPF hens are used as test animals, the environment temperature is controlled to be 10-15 DEG C in the whole test period two days before infection, H9 subtypeAIV and poultry source escherichia coli virulent strains and IBV M41 strains are adopted for sequential co-infection through a nasal inhalation mode, the infected hens show obvious fevering, droopingspirit and cough and asthma symptoms, autopsied diseased hens have characteristic lesions of the H9 subtype avian influenza, and the model is stable and reliable. By means of the method, the obtaineddisease model is stable and typical, and the important basis is laid for subsequently carrying out the avian influenza medicine prevention and cure research.

Description

Technical field: [0001] The invention relates to the technical field of constructing livestock and poultry disease models, in particular to a method for constructing a chicken H9 subtype avian influenza artificial infection model. Background technique: [0002] Avian influenza (AI) is an acute contagious disease caused by influenza A virus (AIV) infection that seriously harms the poultry industry and brings huge economic losses to the poultry industry. Biosecurity and immunization are currently the main means of prevention and control. However, because AIV is easy to mutate, has many subtypes, and the cross-immune protection between each subtype is weak, the phenomenon of immune failure occurs frequently, which brings difficulties to the immune prevention of the disease. The development of effective drugs for the prevention and treatment of bird flu plays an important role in the control of bird flu. In the research and development of new veterinary drugs, successfully con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K35/76
Inventor 赵增成林树乾李桂明宋敏训黄中利杨世发傅剑
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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