Dendranthema indicum extract and application
A technology of Shennong Xiangju and its extract, applied in the biological field, can solve the problems such as the uric acid-lowering effect of Shennong Xiangju which has not been reported, and achieve the effect of good medicinal efficacy and application prospect.
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Embodiment 1
[0025] Example 1 verifies the effect of Shennong Fragrant Chrysanthemum Extract on inhibiting xanthine oxidase activity
[0026] 1. Sample preparation: take the medicinal dregs after extracting volatile oil from Shennong Xiangju as raw material, dry and pulverize them, add 8 times of 75% ethanol, stir, heat and reflux for extraction for 1.5 hours, separate solid and liquid, collect the filtrate, and further add 8 Double the amount of 75% ethanol; stir and heat at 50-60°C for reflux extraction for 1.5 hours, separate the solid and liquid, and collect the filtrate; combine the filtrate, concentrate under reduced pressure to remove ethanol, extract with petroleum ether and ethyl acetate sequentially, and concentrate the ethyl acetate layer to obtain Extract; the ethyl acetate part was separated by MCI gel resin column, eluted with 30%, 75%, and 95% ethanol respectively, and the eluents were collected, concentrated and dried to obtain component A, component B, and component C. It ...
Embodiment 2
[0038] Example 2 Verification of the Uric Acid-lowering Activity of Shennong Fragrant Chrysanthemum Extract
[0039] In this example, using allopurinol as a model control, an animal experiment on reducing uric acid in vivo was carried out to verify the uric acid-lowering activity of the extract of Shennong Fragrant Chrysanthemum.
[0040] (1) Weigh an appropriate amount of potassium oxonate, suspend it with 0.5% CMC-Na solution, and prepare a 12.5 mg / mL modeling solution (wherein, CMC-Na is sodium carboxymethyl cellulose, which is natural cellulose The cellulose derivative obtained after chemical modification is a kind of carboxymethyl etherification of cellulose).
[0041] (2) Take 60 test mice (Kunming species, male mice) and randomly divide them into 6 groups according to body weight, 10 in each group, and set them as blank control group, model control group, allopurinol group, and Shennong Xiangju group respectively. The high-dose group of effective parts, the middle-dose...
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