SNP molecular marker related to pig litter traits, identification of litter trait and combined application thereof

A technology for traits and litter size, applied in the field of pig breeding, can solve problems such as insufficient understanding and understanding, incomplete gene mastery, etc., and achieve the effect of improving the theoretical level and technical level.

Active Publication Date: 2018-09-18
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current understanding and understanding of the pig genome is far from enough, and the genes that affect the re

Method used

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  • SNP molecular marker related to pig litter traits, identification of litter trait and combined application thereof
  • SNP molecular marker related to pig litter traits, identification of litter trait and combined application thereof
  • SNP molecular marker related to pig litter traits, identification of litter trait and combined application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1 Obtaining of SNP molecular markers

[0094] 1. Experimental sample collection

[0095]234 Large White pigs from a pig breeding farm in Shandong were used for SNP typing of DHRS4 gene. Collect ear tissue samples one by one, put them in 75% alcohol and store them at -20°C for DNA extraction.

[0096] 2. DHRS4 gene SNP typing

[0097] 2.1 Extraction and detection of pig genomic DNA

[0098] (1) Cut an appropriate amount of pig ear tissue, cut it into pieces and put it into a 1.5mL Axgen tube;

[0099] (2) Take a 50mL BD centrifuge tube, mix proteinase K with a final concentration of 0.4mg / mL and lysis buffer evenly, and add 0.5mL lysis solution to the 1.5mL centrifuge tube containing pig ear tissue for lysis;

[0100] (3) Place the centrifuge tubes in parallel and evenly on the shaking plate of the thermostatic hybridization furnace (seal the tube caps tightly to avoid liquid leakage). Place at 55°C for more than 6 hours (it is very important to fully mix th...

Embodiment 2

[0133] Example 2 Detection of DHRS4 gene expression

[0134] 1. Experimental sample collection

[0135] The large white pigs and Meishan pigs used to detect the expression of DHRS4 gene came from the pig farm of Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Wuqing District, Tianjin. The ovaries of sows at 180 days and 300 days after birth were collected, and the samples were stored in liquid nitrogen, and total RNA was extracted. Three individuals of each breed were collected as biological replicates. Among them, Meishan pig is one of the breeds with the strongest fecundity and the largest litter size among pig breeds in my country, and its litter size is 30% to 40% higher than that of modern European pig breeds such as Large White pigs. Comparing the expression of DHRS4 gene between Meishan pig and Large White pig can effectively prove the effect of DHRS4 gene expression on litter size. 2. Detection of DHRS4 gene e...

Embodiment 3

[0161] Example 3 Detection of DHRS4 gene-related protein expression level indicators

[0162] 1. Tissue sample processing and total protein quantification

[0163] Tissue sample processing: Take out the ovarian tissue from liquid nitrogen, add 100μL RIPA lysate, and add DTT and PMSF at a final concentration of 1mM just before use. Grind the tissue with a grinding pestle, freeze and thaw several times in liquid nitrogen to fully lyse the cells; place on ice for 30 minutes, then centrifuge at 12000g for 30 minutes at 4°C, and collect the supernatant;

[0164] Total protein quantification: operate according to the instructions of the BCA protein quantification kit, the standard preparation is shown in Table 7, and 10 μL is added after the sample is diluted 10 times. Read values ​​at 570nm on a microplate reader. Draw the standard curve with A570 as the ordinate and the concentration of the standard as the abscissa. The concentration of the protein sample is obtained from the st...

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PUM

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Abstract

The invention discloses SNP loci related to pig litter traits in a DHRS4 gene. The SNP loci are located at Chr7:75253401 (rs326982309) of a pig reference genome Sscrofa11.1 and Chr7:75245594 (rs701332503) of a reference genome Sscrofa11.1. The SNP locus rs326982309 is related to the even birth weight of primiparous piglets of the pig, and the SNP locus rs701332503 is related to the multiparity litter size of the pig. The two SNP loci and determination of relation thereof with the pig litter traits can be effectively used for identification or assisted identification of pig breeds with high litter traits, thereby improving the production level and economic benefits of the pig industry.

Description

technical field [0001] The invention relates to the field of pig breeding, in particular to SNP molecular markers related to pig farrowing traits for improving pig reproduction efficiency, identification and combined application thereof. Background technique [0002] Reproductive performance is one of the key factors affecting the economic benefits of pig farming. As a perennial viviparous animal, a very important indicator of pigleting traits is the litter size, especially the litter size. The litter size can be directly related to the number of fattening pigs and the amount of pork supply. [0003] Another very important indicator of sow farrowing traits is the ability to produce heavier birth weights. The average birth size is large and the coefficient of variation is small, which means that the litter is well-organized, which is very good for improving the survival rate of piglets. The average birth weight can also stimulate sows to produce more milk. The birth weight ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/166
Inventor 周荣刘颖李奎
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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