A method for rapidly creating apomictic flax seeds

A technology of apomictic reproduction and flax seeds, applied in the biological field, can solve the problems of heavy detasseling work, low success rate, cumbersome methods, etc.

Active Publication Date: 2021-09-28
黑龙江瑞家生物医药科技有限公司
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Problems solved by technology

There have been methods to prove that apomictic seeds can be obtained by using polyembryonic flax germplasm (Kang Qinghua, 2014; patent: ZL 2013 1 0355880.X), but this method requires the use of 2.4-D and inactivated pollen. Methods to induce polyembryonic flax to obtain apomictic seeds ("Preliminary Research on the Induction of Apomictic Growth Regulators in Flax", China Hemp Industry Science, Kang Qinghua, 2013), the method is cumbersome, the detasseling work is heavy, and the concentration of the inducing agent is strict. low success rate

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  • A method for rapidly creating apomictic flax seeds
  • A method for rapidly creating apomictic flax seeds

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Embodiment 1

[0032] Embodiment 1, the method utilizing polyembryonic resource and nuclear sterile material to create apomictic linseed

[0033] 1. Obtain the M0 generation seed of hybrid fruit

[0034]In July 2015, the male sterile flax germplasm M1401 was used as the female parent, and the polyembryonic flax germplasm 2008DH08007(1) was used as the male parent, and cross-pollinated to obtain the polyembryonic male sterile hybrid combination M1503; in April 2016 The seeds of M1503 were planted in the field last month, and recorded as 2016KDZ188. During the flowering period of this material, 6 sterile plants were found (no pollen or anthers were white and shriveled), and the rate of multiple embryos in the seeds of fertile plants was 0.1%. The obtained male sterile flax germplasm 2016KDZ188 has dual gene characteristics of polyembryony and sterility.

[0035] In June 2016, at the flowering stage of the male sterile flax germplasm 2016KDZ188, observation began, and all male sterile plants w...

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Abstract

The invention discloses a method for rapidly creating apomictic flax seeds. The invention provides a method for creating apomictic flax seeds, comprising the following steps: 1) using polyembryonic flax germplasm with apomictic characteristics as the male parent, and using sterile plants of male sterile flax germplasm The female parent is hybridized, and the hybrid fruit is harvested; 2) the seeds of the hybrid fruit are sown, the sterile plants are selected and bagged without pollination, and the seeds in the harvested fruit are obtained to obtain apomictic flax seeds. The method of the invention can simplify work such as castration, hormone induction and inactivated pollen stimulation in the method for obtaining apomictic seeds, and improves the working efficiency and accuracy of obtaining apomictic flax seeds. Therefore, on the basis of utilizing the polyembryonic flax germplasm, the present invention adopts the method of introducing the male sterility gene, which can simplify the operation procedure and obtain apomictic flax seeds quickly and accurately.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapidly creating apomictic flax seeds. Background technique [0002] In the past 50 years, the production potential of major crops in the world has been clearly brought into play by the full use of heterosis. Apomixis has the advantages of fixing heterosis, accelerating the breeding process, and accumulating good genes. Therefore, apomixis can be used to quickly fix the heterosis of crops and achieve improved It will have great significance and potential in agricultural production and genetic breeding (Wu Man et al., 2010). Because of its advantages of fixing heterosis, accelerating breeding process, and accumulating good genes, apomixis has great significance and potential in agricultural production and genetic breeding [Wu Man et al., 2010]. Although apomictic plants have been found in more than 400 species in 52 families of angiosperms, apomictic traits are generally...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/10A01H6/58
CPCA01H1/02A01H1/04A01H5/10
Inventor 康庆华王玉富张树权姜卫东宋喜霞孙中义吴广文袁红梅徐涵谢冬微于莹姚玉波黄文功陈晶胡莹莹刘岩程莉莉
Owner 黑龙江瑞家生物医药科技有限公司
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