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Cloning primers of pig PLIN1 gene, single nucleotide polymorphic site detection method and application

A single nucleotide polymorphism, PLIN1 technology, applied in the field of molecular biology, can solve the problem of rare research on the function of porcine PLIN gene, and achieve the effect of improving pork quality, good specificity and rapid identification

Active Publication Date: 2018-09-28
HEBEI UNIV OF ENG +1
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  • Abstract
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Problems solved by technology

Although the physiological function of the PLIN gene in humans and other animals is relatively clear, there are few studies on the function of the pig PLIN gene

Method used

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  • Cloning primers of pig PLIN1 gene, single nucleotide polymorphic site detection method and application
  • Cloning primers of pig PLIN1 gene, single nucleotide polymorphic site detection method and application
  • Cloning primers of pig PLIN1 gene, single nucleotide polymorphic site detection method and application

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Embodiment Construction

[0040] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0041] A kind of cloning primer of porcine PLIN1 gene, described primer is Exon3, comprises forward primer and reverse primer, and sequence is as follows:

[0042] Forward primer F: GGGAAGGCACTGCTCTGG,

[0043] Reverse primer R: CTTCACTTGGCCCCACAG.

[0044] A kind of cloning primer of porcine PLIN1 gene, described primer is Exon5, comprises forward primer and reverse primer, and sequence is as follows:

[0045] Forward primer F: ATCCACCCTCACCGACCC,

[0046] Reverse primer R: GGATGCTTAACCCCCCGT.

[0047] A method for detecting a single nucleotide polymorphism site of a pig PLIN1 gene, comprising:

[0048] aUsing pig genomic DNA as a template, using any cloning primer of Exon3 or Exon5 to carry out PCR amplification to obtain PCR amplification products;

[0049] b. Recover and detect the PCR amplification products,...

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Abstract

The invention belongs to the technical field of molecular biology, provides pig PLIN1 gene cloning primers, a single nucleotide polymorphic site detection method and application and discloses two pairs of primers for cloning pig PLIN1 gene. The primers are utilized for PCR amplification by taking pig genome DNA as a template, a PCR amplification product is recycled and detected before mixed pool sequencing is performed, a pig PLIN1 gene sequencing result is compared with a published target sequence, and after the target sequence is confirmed, comparison with DNA of pigs of other different varieties is performed to judge a polymorphic site. By adopting a PCR method, a sequence of the pig PLIN1 gene is cloned successfully, single nucleotide polymorphic site detection is performed, and the primers are used for variety identification.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a cloning primer of a porcine PLIN1 gene, a method for detecting a single nucleotide polymorphism site and an application thereof. Background technique [0002] China has been a big country in pig production and pork consumption since ancient times, and pork occupies an extremely important position in people's daily life. People not only have a large demand for pork, but with the continuous development of the times, people's consumption level and quality of life are increasing day by day, and the requirements for pork quality are getting higher and higher. Nowadays, the breeding direction of pigs has undergone a transformation from high-fat pork to lean-meat pigs all over the world. In addition to improving the reproductive performance, growth rate, and meat output of pigs, how to improve the pig breeding direction and production practice? , to achieve a ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12Q1/6888C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113
Inventor 白莹董新星许桥杨俊琦李明丽刘玉芳包立兰国湘张鑫
Owner HEBEI UNIV OF ENG
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