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Cloning primers, single nucleotide polymorphism site detection method and application of porcine plin1 gene

A single nucleotide polymorphism, PLIN1 technology, applied in the field of molecular biology, can solve the problem of rare research on the function of porcine PLIN gene, and achieve the effect of improving pork quality, good specificity and rapid identification

Active Publication Date: 2022-06-07
HEBEI UNIV OF ENG +1
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  • Abstract
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Problems solved by technology

Although the physiological function of the PLIN gene in humans and other animals is relatively clear, there are few studies on the function of the pig PLIN gene

Method used

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  • Cloning primers, single nucleotide polymorphism site detection method and application of porcine plin1 gene
  • Cloning primers, single nucleotide polymorphism site detection method and application of porcine plin1 gene
  • Cloning primers, single nucleotide polymorphism site detection method and application of porcine plin1 gene

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Embodiment Construction

[0040] The following will be further elaborated in conjunction with the accompanying drawings and embodiments of the technical solution of the present invention.

[0041]A clone primer of the porcine PLIN1 gene, said Exon3, consisting of forward primers and reverse primers, in the following sequence:

[0042] Forward primer F: GGGAAGGCACTGCTCTGG,

[0043] Reverse primer R: CTTCACTTGGCCCCACG.

[0044] A cloned primer of the porcine PLIN1 gene, said Exon5, consisting of forward and reverse primers, in the following sequence:

[0045] Forward primer F: ATCCACCCTCACCGACCC,

[0046] Reverse primer R: GGATGCTTAACCCCGT.

[0047] A method for detecting single nucleotide polymorphism sites in porcine PLIN1 gene, including:

[0048] a PcR amplification products were obtained by using either Exon3 or Exon5 clonal primers as a template using either Exon3 or Exon5.

[0049] b The PCR amplification products are recovered, detected, and then sequenced in the mixed pool;

[0050] c Comparing the ...

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Abstract

The invention belongs to the technical field of molecular biology, provides porcine PLIN1 gene cloning primers, a single nucleotide polymorphism site detection method and its application, discloses two pairs of primers for cloning porcine PLIN1 gene, and uses porcine genome DNA as a template, Use primers to carry out PCR amplification, recover and detect the PCR amplification products, and perform mixed pool sequencing, compare the porcine PLIN1 gene sequencing results with the published target sequence, and compare it with the DNA of other different breeds of pigs after confirming that it is the target sequence , to determine the polymorphic site. The invention adopts the PCR method to successfully clone the sequence of the porcine PLIN1 gene, detect the single nucleotide polymorphism site, and use it for the identification of the variety.

Description

Technical field [0001] The present invention belongs to the field of molecular biology technology, specifically related to cloning primers of porcine PLIN1 gene, single nucleotide polymorphism site detection method and application. Background [0002] China has been a big country in pig production and pork consumption since ancient times, and pork occupies an important position in people's daily lives. People not only have a large demand for pork, with the continuous development of the times, people's consumption level and quality of life are growing, and the requirements for pork quality are getting higher and higher. Nowadays, the breeding direction of pigs has experienced the transformation of high-fat pork to lean pig breeding in the world, in addition to improving the reproductive performance, growth rate, meat yield, etc. of pigs, how to achieve a balance between "meat quality" and "meat production" in the pig breeding direction and production practice is a hot issue in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858C12Q1/6888C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113
Inventor 白莹董新星许桥杨俊琦李明丽刘玉芳包立兰国湘张鑫
Owner HEBEI UNIV OF ENG
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