Breeding method for new varieties of Dendrobium orchid
A new variety, the technology of dendrobium orchid, is applied in the field of new variety breeding of dendrobium orchid, which can solve the problems of long cultivation period and achieve the effect of short cultivation period and high survival rate
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[0013] A kind of dendrobium orchid new variety breeding method, comprises the steps:
[0014] Step 1, select the lateral buds and terminal buds of Dendrobium nobile orchid with excellent flower shape and color as explants, and cultivate them in the mutagenesis medium for 40 days to form protocorms; the formula of the mutagenesis medium is: 5 g of sucrose, peptone 10g, 2.5g of agar powder, 1.5g of ethyl methanesulfonate, 0.2g of clomazone, and make up to 1L of water; the culture temperature is 20-25°C, the light intensity is 1500-1800lx, and the light time is 12h / day;
[0015] Step 2, the protocorm-like continues to develop, and some protocorm-like plants develop into plantlets, and the protocorm-like plants that form the plantlets are transferred to the proliferation medium for value-added differentiation and culture for about 30 days to form seedlings; the formula of the proliferation medium is: Huabao No. 1 1g, Huabao No. 2 1g, peptone 5g, coconut juice 2g, sucrose 20g, agar...
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