Specific SSR primers for malus sieversii and cultivated apple and applications of specific SSR primers

A specific, apple technology, applied to the specific SSR primers of Xinjiang wild apple and cultivated apple and its application field, can solve the problem that the function of the target band and the encoded protein are unclear, and the specific sequence of genetic differences and the biological role are difficult to determine. , difficult to determine, etc.

Active Publication Date: 2018-10-16
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The analysis of genetic diversity and phylogeny of wild apple in Xinjiang is mainly based on SSR primers, but the target fragments amplified by SSR primers currently used for the analysis of genetic diversity in wild apple in Xinjiang are all non-specific amplification. The protein is not clear, and the specific sequence and biological role of the c

Method used

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  • Specific SSR primers for malus sieversii and cultivated apple and applications of specific SSR primers
  • Specific SSR primers for malus sieversii and cultivated apple and applications of specific SSR primers
  • Specific SSR primers for malus sieversii and cultivated apple and applications of specific SSR primers

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Embodiment 1

[0102] Embodiment 1 Amplifies the design and screening of specific SSR primers for specificity, stability and polymorphic SSR target bands in wild apples and cultivated apples in Xinjiang

[0103] 1 Materials and methods

[0104] 1.1 Sample information for screening SSR primers

[0105] Wild apples in Xinjiang were collected from Ili, Xinjiang from August 2016 to September 2017, and cultivated apples were collected from Yantai and Qingdao in Shandong from July to August 2017. The sample information is shown in Table 1 and Table 2; the genome DNA extraction of wild apples and cultivated apples in Xinjiang was carried out in strict accordance with the kit instructions.

[0106] Table 1 Sample information sheet

[0107] serial number

Sample serial number

sampling time

sampling location

storage method

Variety

1

LY-1

2017.7.30

Shandong Laiyang

Silica gel drying

Xiao Guoguang

2

LS-1

2017.8.4

Shandong Laoshan

...

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Abstract

The invention discloses specific SSR primers for malus sieversii and the cultivated apple and applications of the specific SSR primers. Based on the whole genome sequence of the cultivated apple, a functional gene sequence relevant to the stress resistance of apple is adopted as a template, and 15 pairs of SSR primers amplifying SSR target bands with specificity, stability and polymorphism in malus sieversii and the cultivated apple are screened, wherein the nucleotide sequences of the SSR primers are as shown in SEQ ID No.1-30. 8 pairs of SSR primers only capable of amplifying SSR target bands in the cultivated apple with specificity are also screened. The protein products coded by the target bands amplified by the SSR primers are known, and the SSR primers can be used for carrying out genetic diversity, polymorphis or phylogenetic analysis on the malus sieversii and cultivated apple, thus disclosing the genetic diversity and phylogenetic relationship of the malus sieversii and cultivated apple and the influences of the obtained genetic variation on the malus sieversii and cultivated apple in the aspects including the biological function.

Description

technical field [0001] The present invention relates to SSR primers, in particular to specific SSR primers designed based on the genome of cultivated apples that can amplify specific, stable and polymorphic SSR target bands in Xinjiang wild apples and cultivated apples. The present invention further relates to The application of the specific SSR primer in the analysis of Xinjiang wild apple and cultivated apple genetic diversity, polymorphism analysis or phylogenetic analysis belongs to the field of apple specific SSR primer and its application. Background technique [0002] Xinjiang wild apple (Malus sieversii (Ledeb.) Roem.) belongs to the Tertiary relict plant, which may be the ancestor species of modern cultivated apple, and is an important natural germplasm gene bank for the sustainable development of forest fruit industry. In recent years, due to the interference of human activities and the threat of pests such as Agrilus mali Matsumura, the natural population of wild ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11G06F19/24
CPCG16B40/00C12Q1/6895C12Q2600/156
Inventor 于少帅赵文霞姚艳霞淮稳霞肖文发
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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