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Construction of "z"-shaped optoelectronic aptamer analysis method based on dnawalker signal amplification

A technology of signal amplification and analysis method, applied in the field of nanomaterials and biological analysis, it can solve the problems of electron and hole recombination, transfer, etc. Effect

Inactive Publication Date: 2019-07-09
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In photoelectrochemical detection, light is used as an excitation signal to excite the photosensitive material. When the energy of the light absorbed by the photosensitive material is higher than its band gap width, electrons transition from the valence band to the conduction band to generate holes, but the electrons and holes are electrically opposite. Recombination or charge transfer is easy to occur, so increasing the photocurrent to improve sensitivity will reduce the recombination rate of electrons and holes

Method used

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  • Construction of "z"-shaped optoelectronic aptamer analysis method based on dnawalker signal amplification
  • Construction of "z"-shaped optoelectronic aptamer analysis method based on dnawalker signal amplification
  • Construction of "z"-shaped optoelectronic aptamer analysis method based on dnawalker signal amplification

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Embodiment 1

[0036] 1. Synthesis method of carboxylated CdS quantum dots and BiVO 4Preparation method of gold nanoparticles deposited on {010} crystal surface 0.175 mmol CdCl 2 2.5 H 2 Dissolve O and 0.289 mmol thiourea in 14 mL water, add 0.395 mmol mercaptopropionic acid to 34 mL, adjust the pH to 10 with 1M NaOH solution, put it into a 100 mL reaction vessel, and heat at 100 °C for 120 minutes, the resulting quantum dot lens image Such as figure 2 As shown in (A), the average particle size is 3.5 nm.

[0037] 18 mmol Bi(NO 3 ) 3 ·5H 2 O with an equivalent amount of NH 4 VO 3 Dissolve in 150 ml of 2M nitric acid, stir and add ammonia water to adjust the pH to 2, continue to stir until the solution turns orange, after aging for 2 hours, transfer the precipitate to a 50 mL reactor and heat at 200 degrees for 24 hours to obtain BiVO 4 , centrifugal washing and drying at 60°C. Take 250 mg BiVO 4 Add 50 mL of water and sonicate for 1 hour, adding a mass fraction of BiVO 4 5% chl...

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Abstract

The invention discloses an analysis method of a constructed Z-shaped photoelectric adaptor based on DNA Walker signal amplification. The method is inspired by plant photosynthesis Z electron transfer,and connects a CdS quantum dot to nanogold deposited to BiVO4 through DNA Walker, and photo-induced electron produced by the BiVO4 is transferred to a valence band of the CdS quantum dot from a conduction band of the BiVO4 through electron transfer effect of nanogold, so that compounding of electrons and electron holes is inhibited, photoelectric current is improved, and signal amplification is realized. The method adopts an electrochemical workstation combined with a xenon lamp, is low in cost, high in detection sensitivity, applies bionics to photoelectrochemistry, and provides a sensitivephotoelectrochemistry detection method with stable performances for the detection of cancer markers.

Description

technical field [0001] The invention relates to an analysis method for constructing a "Z" photoelectric aptamer based on DNA Walker signal amplification, and belongs to the technical field of nanomaterials and biological analysis. Background technique [0002] At present, the incidence of cancer in my country is showing an increasing trend year by year, and the mortality rate remains high. This is closely related to the fact that many patients are already in the middle and late stages when they are diagnosed. The treatment effect of middle and late cancers will be greatly weakened compared with early stages. Treatment is very important. [0003] As a new type of electrochemical biosensor, photoelectrochemical biosensor has the advantages of low price, simple equipment, rapid response, and sensitive detection. Compared with the traditional ECL and kit detection methods used in hospitals, its outstanding feature is that it has Higher sensitivity, which is of great significance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3278
Inventor 唐点平吕姝臻张康耀邱桢丽舒键
Owner FUZHOU UNIV
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